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Merck

G6795

モノクローナル抗緑色蛍光タンパク質(GFP), N末端抗体 マウス宿主抗体

clone GSN24, purified from hybridoma cell culture

別名:

Anti-GFP

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この商品について

NACRES:
NA.56
UNSPSC Code:
12352203
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製品名

モノクローナル抗緑色蛍光タンパク質(GFP), N末端抗体 マウス宿主抗体, clone GSN24, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GSN24, monoclonal

form

buffered aqueous solution

mol wt

antigen 27 kDa

concentration

~2 mg/mL

technique(s)

western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C
2-8°C

target post-translational modification

unmodified

Quality Level

Biochem/physiol Actions

GFP融合タンパク質と特異的に反応します。
Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.

Physical form

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)

Application

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

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保管分類

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Design of inducible expression vectors for improved protein production in Ralstonia eutropha H16 derived host strains
Gruber S, et al.
Journal of Biotechnology, 235(8), 92-99 (2016)
Diggin? on U (biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates
Rubel CE, et al.
Cell Biochemistry and Biophysics, 67(1), 127-138 (2013)
Marta Ukleja et al.
Nature communications, 7, 10433-10433 (2016-01-26)
CCR4-NOT is a large protein complex present both in cytoplasm and the nucleus of eukaryotic cells. Although it is involved in a variety of distinct processes related to expression of genetic information such as poly(A) tail shortening, transcription regulation, nuclear
Hirofumi Aso et al.
Cell reports, 32(2), 107887-107887 (2020-07-16)
For eradication of HIV-1 infection, it is important to elucidate the detailed features and heterogeneity of HIV-1-infected cells in vivo. To reveal multiple characteristics of HIV-1-producing cells in vivo, we use a hematopoietic-stem-cell-transplanted humanized mouse model infected with GFP-encoding replication-competent HIV-1. We
Mohammad I K Hamad et al.
Development (Cambridge, England), 141(8), 1737-1748 (2014-03-29)
The ionotropic α-amino-3-hydroxy-5-methyl-4-isoxazole propionate glutamate receptors (AMPARs) have been implicated in the establishment of dendritic architecture. The transmembrane AMPA receptor regulatory proteins (TARPs) regulate AMPAR function and trafficking into synaptic membranes. In the current study, we employ type I and

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Instructions

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