G6795
モノクローナル抗緑色蛍光タンパク質(GFP), N末端抗体 マウス宿主抗体
clone GSN24, purified from hybridoma cell culture
別名:
Anti-GFP
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この商品について
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
GSN24, monoclonal
Application:
WB
Citations:
39
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GSN24, monoclonal
form
buffered aqueous solution
mol wt
antigen 27 kDa
concentration
~2 mg/mL
technique(s)
western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C, 2-8°C
target post-translational modification
unmodified
Quality Level
関連するカテゴリー
General description
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.
Application
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.
Biochem/physiol Actions
GFP融合タンパク質と特異的に反応します。
Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.
Physical form
0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
G6795-VAR: + G6795-200UG: + G6795-BULK:
jan
Marta Ukleja et al.
Nature communications, 7, 10433-10433 (2016-01-26)
CCR4-NOT is a large protein complex present both in cytoplasm and the nucleus of eukaryotic cells. Although it is involved in a variety of distinct processes related to expression of genetic information such as poly(A) tail shortening, transcription regulation, nuclear
Diggin? on U (biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates
Rubel CE, et al.
Cell Biochemistry and Biophysics, 67(1), 127-138 (2013)
Design of inducible expression vectors for improved protein production in Ralstonia eutropha H16 derived host strains
Gruber S, et al.
Journal of Biotechnology, 235(8), 92-99 (2016)
Meenakshi Verma et al.
PloS one, 7(8), e42923-e42923 (2012-08-11)
Small molecules with antioxidative properties have been implicated in amyloid disorders. Curcumin is the active ingredient present in turmeric and known for several biological and medicinal effects. Adequate evidence substantiates the importance of curcumin in Alzheimer's disease and recent evidence
Hirofumi Aso et al.
Cell reports, 32(2), 107887-107887 (2020-07-16)
For eradication of HIV-1 infection, it is important to elucidate the detailed features and heterogeneity of HIV-1-infected cells in vivo. To reveal multiple characteristics of HIV-1-producing cells in vivo, we use a hematopoietic-stem-cell-transplanted humanized mouse model infected with GFP-encoding replication-competent HIV-1. We
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Instructions
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