Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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その他の試薬を追加 (MAPmatesとともに使用するにはバッファーおよび検出キットが必要です)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option(チェックを入れると箱詰めから袋詰めに変更となりますのでご注意ください) Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
High Performance Spherical Silica for Efficient Peptide Purification
PharmPrep™ P is a spherical, porous silica sorbent available in two particle sizes: 10 and 20 μm for preparative liquid chromatography (LC). With a pore diameter of 100Å (10 nm), these sorbents fit perfectly into the polishing step of small peptides like insulin, and other biopharmaceutical and active pharmaceutical ingredients (APIs) like antibiotics and hormones.
PharmPrep™ P sorbents are characterized by:
Uniform and homogenous silica gel matrix with excellent batch-to-batch reproducibility over many column packings
Narrow particle size distribution for high performance and high productivity in small peptide purification processes
Reproducible specific surface area and pore size distribution
Low back pressure
Enhanced mechanical stability for long sorbent life-time
Superior polishing of insulin
PharmPrep™ P sorbent demonstrates a perfect separation of insulin and the main impurities desamido-insulin. Using PharmPrep™ P sorbent in your polishing step, an outstanding insulin purity of 99.8% can be achieved.
(A) Process separation, (B) In-process control on insulin production (54.5-58.0 min) Purospher® STAR 5 µm column. Click image to enlarge
PharmPrep™ P 100 RP-18e, 10 μm sorbent chromatographic conditions for polishing of crude human insulin (90% pure)
Column Size
250 x 4.6 mm
Mobile phase
A: 0.1 M NH4H2PO4 pH 7.3 B: 0.1 M NH4H2PO4 pH 7.3/ACN 5/5 (Gradient mode 65% A–30% A 100 min)
Flow rate
1.325 mL/min
Temp.
25 °C
Detection
UV 214 nm
Injection volume
100 μL
Sample
18 mg/mL crude human Insulin rec. dissolved in water +0.1% TFA
Three types of PharmPrep™ P Sorbents are available: