Reinforced Clostridial Medium (RCM)

Appearance (clearness): clear
Appearance (colour): yellowish
pH-value (25 °C): 6.6 - 7.0
Typical composition (g/litre): Meat extract 10.0; Peptones 10.0; Yeast extract 3.0; D(+)Glucose 5.0; Starch 1.0; Sodium chloride 5.0; Sodium acetate 3.0; L-Cysteinium chloride 0.5; Agar-agar 0.5
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Clostridium sporogenes ATCC 11437): ≤ 100
Inoculum on reference medium (Clostridium sporogenes ATCC 19404 (WDCM 00008)): ≤ 100
Inoculum on reference medium (Clostridium perfringens ATCC 10543): ≤ 100
Inoculum on reference medium (Clostridium novyi ATCC 17861): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Growth (Clostridium sporogenes ATCC 11437): +
Growth (Clostridium sporogenes ATCC 19404 (WDCM 00008)): +
Growth (Clostridium perfringens ATCC 10543): +
Growth (Clostridium novyi ATCC 17861): +
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): +
Incubation: 48 hrs.; 30-35°C; Clostridia: anaerobic
The Information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.

GranuCult^® prime Reinforced clostridial medium (RCM) is suitable for the cultivation and enumeration of clostridia, other anaerobes, and facultative microorganisms in foodstuffs and other materials.

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

GranuCult^® prime Reinforced clostridial medium (RCM) be used to initiate growth from small inoculum and to obtain the highest viable count of Clostridia. It can be used as the conventional medium for studies of spore-forming anaerobes, especially Clostridium butyricum in cheese, and for the enumeration and isolation of Clostridia. Other spore-forming anaerobes like streptococci and lactobacilli grow as well on this media. Casein hydrolysate, beef extract, yeast extract, and peptone provide nitrogen, vitamins, amino acids, and carbon for growth. Dextrose is a fermentable sugar, and sodium chloride ensures osmotic balance. The medium is free from inhibitors and contains cysteine as a reducing agent. Sodium acetate is the buffering agent. Polymyxin B can be added to inhibit Gram-negative bacteria and to make the medium more selective. Culture conditions include incubation at 35-37°C for 40-48 hours in an anaerobic atmosphere.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany