Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

17.5 kDa

Fibroblast growth factor 2 (UniProt: P03969; also known as FGF-2, basic fibroblast growth factor (bFGF), Heparin-binding growth factor 2 (HBGF-2)) is encoded by the FGF2 gene (Gene ID: 281161) in bovine. Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm patterning in the early embryonic development. In adults, FGFs participate in several physiological and pathological processes, including cell proliferation, cell survival, differentiation, wound healing, and regulation of angiogenesis. FGF-2 is synthesized with a propeptide that is subsequently cleaved off to generate the mature active growth factor that can act as a monomer or a homodimer to act as a ligand for FGFR1, FGFR2, FGFR3, and FGFR4. Following its synthesis, it is exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF-2 occurs by direct translocation across the plasma membrane. It is shown that binding of exogenous FGF-2 to FGF receptors facilitates endocytosis followed by translocation of FGF-2 across endosomal membrane into the cytosol. Its import into nucleus requires the classical nuclear import machinery, involving Karyopherin a1 and b1 and the centrosomal protein 57 (CEP57). FGF-2 expression is observed in granulosa and cumulus cells. It is also expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. FGF-2 is also reported to act as an integrin ligand and binds to integrin aV:b3. This bFM-1 mouse monoclonal antibody recognizes the non-denatured form of FGF-2/basic FGF and is recommended for neutralizing activity. (Ref.: Matsuzaki, K., et al. (1989). Proc Natl Acad Sci USA. 86(24):9911-5; Shimizu, T., et al. (2017). JCI Insight. 2(13):e93187; Fukuoka, M., et al. (2018). PLoS One. 13(8):e0201796; Tisi, A., et al. (2020). Sci Rep. 10(1):6376)

Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.

Anti-FGF-2/basic FGF (neutralizing), clone bFM-1, Cat. No. 05-117, is a mouse monoclonal antibody that detects Basic fibroblast growth factor (bFGF) and is tested for use in ELISA, Western Blotting, Radioimmunoassay, and Neutralizing.

Tested Applications

Neutralizing Assay: A representative lot of this antibody prevented the proliferation of fibroblasts and neutralized the biological activity of BK1 (bFGF1-54; KGF91-194) and BK2 (bFGF1-74; KGF 111-194) chimeras. (Reich-Slotky, R., et al. (1995). J Biol Chem. 270(50):29813-8; Matsuno, Y., et al. (2022). Sci Rep. 12(1):12519).

Radioimmunoassay: A representative lot of this antibody detected basic fibroblast growth factor (bFGF) in Radioimmunoassay (Matsuzaki, K., et al. (1989). Proc Natl Acad Sci USA. 86(24):9911-5).

Western Blotting Analysis: A representative lot detected Basic fibroblast growth factor (bFGF) in Western Blotting application (Tisi, A., et al. (2020). Sci Rep. 10(1):6376).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone bFM-1 is a mouse monoclonal antibody that specifically detects FGF-2/basic FGF. This highly specific antibody does not cross-react with bovine acidic FGF (FGF-1, HBGF-1). This antibody recognizes the non-denatured form of FGF-2/basic FGF.

Format: Purified

Purified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.

Evaluated by ELISA with recombinant human Basic fibroblast growth factor.

ELISA: A representative lot of this antibody detected Basic fibroblast growth factor (bFGF) protein in ELISA application.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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