Anti-E2F-1 Antibody, clones KH20 and KH95

55 kDa

E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Epitope: The epitope for KH20 has been mapped to amino acids 1-89; KH95 to amino acids 342-386.

GST fusion protein corresponding to full-length human E2F-1.

Anti-E2F-1 Antibody, clones KH20 & KH95 is a high quality Mouse Monoclonal Antibody for the detection of E2F-1 & has been validated in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Recognizes E2F-1.

Format: Purified

Protein G Purified

Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide.

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunoblot Analysis: 0.5-2μg/ml of this antibody detected E2F-1 in a HeLa nuclear extract.
Immunoprecipitation: 4μg of this antibody immunoprecipitated E2F-1 from HeLa nuclear extract.
Gel Shift: The KH20 clone has been shown to gel shift the E2F-1/DP-1/DNA complex (Helin, 1993; Wu, 2000).

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.