05-633
Anti-BrdU Antibody, clone BU-1
culture supernatant, clone BU-1, Upstate®
동의어(들):
Anti-BrdU antibody
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
BU-1, monoclonal
Technique(s):
immunocytochemistry: suitable
Application:
ICC
Citations:
20
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
BU-1, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
isotype
IgG2a
shipped in
wet ice
target post-translational modification
unmodified
General description
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
none given
Immunogen
5-iodouridine:ovalbumin conjugate
Application
Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC.
Biochem/physiol Actions
5-Bromo-2-deoxyuridine
Physical form
4 vials, each vial containing mouse culture supernatant lyophilized from 2.5 mL. The supernatant contains mycoplasma, which contribute enzymes that facilitate antibody binding. Lyophilized powder.
Analysis Note
A representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316).
Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
Tudor, CS; Bruns, H; Daniel, C; Distel, LV; Hartmann, A; Gerbitz, A; Buettner, MJ
Testing null
N J Gonchoroff et al.
Cytometry, 6(6), 506-512 (1985-11-01)
We describe a mouse monoclonal antibody (BU-1) reactive with 5-bromo-2-deoxyuridine (BrdUrd). The antibody is different from previously described BrdUrd monoclonal antibodies in that BU-1 does not require pretreatment of cells with strong DNA denaturants in order for the antibody to
Difference between random and imprinted X inactivation in common voles.
Elena V Dementyeva,Alexander I Shevchenko,Olga V Anopriyenko,Nina A Mazurok et al.
Chromosoma null
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 05-633 | 04053252368066 |