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Merck

05-844

Anti-PLK1 Antibody, clone 35-206

clone 35-206, Upstate®, from mouse

동의어(들):

Polo-like kinase 1, Serine/threonine-protein kinase 13, cell cycle regulated protein kinase, polo (Drosophia)-like kinase, polo like kinase, polo like kinase, polo-like kinase (Drosophila), polo-like kinase 1 (Drosophila)

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
35-206, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
50

주요 문서

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기술 서비스
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도움 문의

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

35-206, monoclonal

species reactivity

rat, mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG2b

NCBI accession no.

NP_005021

UniProt accession no.

P53350

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100, 400

Gene Information

human ... PLK1(5347)
mouse ... Plk1(18817)

General description

64 kDa
Polo-like Kinase 1 (PLK-1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK-1 protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

Immunogen

6His-tagged full length fusion protein corresponding to human polo-like kinase 1 (PLK1). Also called serine-threonine protein kinase PLK.

Application

Anti-PLK1 Antibody, clone 35-206 detects level of PLK1 & has been published & validated for use in WB, IP, IC, IH.
Immunoprecipitation: An independent laboratory has reported that a previous lot of this antibody can be used to immunoprecipitate PLK1.

Immunohisto/immunocytochemistry: An independent laboratory reported that 1 μg/mL of a previous lot of this antibody showed positive immunostaining for PLK1 in paraformaldehyde treated, Triton X-100 fixed cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

PLK1

Physical form

Format: Purified
Protein A purified
Protein A purified mouse IgG2b in buffer containing 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. Store at -20°C.

Preparation Note

Stable for 1 year at from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Evaluated by western blot on RIPA lysates from 3T3/A31 cells.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected PLK1 in RIPA lysates from 3T3/A31 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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저장 등급

10 - Combustible liquids

wgk

WGK 1

시험 성적서(COA)

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문서 라이브러리 방문

The family of polo-like kinases.
Golsteyn, R M, et al.
Progress in Cell Cycle Research, 2, 107-114 (1996)
Neuronal c-Abl activation leads to induction of cell cycle and interferon signaling pathways.
Schlatterer, SD; Suh, HS; Conejero-Goldberg, C; Chen, S; Acker, CM; Lee, SC; Davies, P
Journal of Neuroinflammation null
Guoning Liao et al.
Scientific reports, 8(1), 12635-12635 (2018-08-24)
Polo-like kinase 1 (Plk1) has been implicated in mitosis, cytokinesis, and proliferation. The mechanisms that regulate Plk1 expression remain to be elucidated. It is reported that miR-100 targets Plk1 in certain cancer cells. Here, treatment with miR-100 did not affect
Chieh-Yuan Cheng et al.
Oncotarget, 9(30), 21231-21243 (2018-05-17)
BI2536 has been developed as a potential therapeutic agent for various cancers but not in oral cancer cells. Since BI2536 exhibits mitosis-regulating activity which are the most radiosensitive, we hypothesized that BI2536 might modulate the radiosensitivity of oral cancer cells.
Hitesh Patel et al.
Journal of molecular cell biology, 8(4), 338-348 (2016-03-20)
Loss of Kindlin 1 (Kin1) results in the skin blistering disorder Kindler Syndrome (KS), whose symptoms also include skin atrophy and reduced keratinocyte proliferation. Kin1 binds to integrins to modulate their activation and more recently it has been shown to
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