Anti-CREB Antibody

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.

~43 kDa observed.

Epitope: Amino acids 5-24.

KLH-conjugated, synthetic peptide (CSGAENQ QSGDAAVTEAENQQ) corresponding to a.a. 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat.

Anti-CREB Antibody, Cat. No. 06-863, is a highly specific rabbit Polyclonal antibody, that targets CREB and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated CREB in 500 µg of HeLa nuclear extract.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected an enhanced CREB occupancy at the LYPD3 promoter in LKB1-deficient TE-4 human esophageal cancer cells (Gu, Y., et al. (2012). Oncogene. 31(4):469–479).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB association at the RNASET2 sites targeted by T-cell Leukemia Virus type 1 (HTLV-1) encoded Tax protein using HTLV-1-infected human T-cell lines, MT-2, C8166/45 and SLB-1 (Polakowski, N., et al. (2011). Viruses. 3(8):1485-500).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB occupancy at the hBDNF promoter IV using postmortem human parietal cortex tissue chromatin preparations (Pruunsild, P., et al. (2011). J. Neurosci. 31(9):3295-3308).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB occupancy at the miR-9-2 promoter in SK-N-BE human neuroblastoma cells (Laneve, P., et al. (2010). Nucl. Acids Res. 38(20):6895-6905).
ChIP-seq Analysis: A representative lot identified CREB target genes in murine spermatogonia derived GC1-spg cells by ChIP-seq analysis (Martianov, I., et al. (2010). BMC Genomics. 11:530).
Western Blotting Analysis: Representative lots detected CREB in lysates from cultured embryonic rat striatal neurons and PC12 pheochromocytoma cells (Hutchinson, A.N., et al. (2012). Neuropsychopharmacology. 37(2):321-337; Maharjan, S., et al. (2010). J. Neurochem. 112(1):42-55).
Western Blotting Analysis: Representative lots detected CREB in mouse proximal caput epididymis-1 (PC-1) cell lysate and hippocampus tissue homogenates (Hamzeh, M., and Robaire, B. (2011). J. Endocrinol. 209(1):55-64; Zhou, Z., et al. (2009). Neuropharmacology. 56(1):81-89).
Western Blotting Analysis: A representative lot detected CREB in SK-N-BE human neuroblastoma cell lysate (Laneve, P., et al. (2010). Nucl. Acids Res. 38(20):6895-6905).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-CREB complex in EMSA using radiolabeled oligonucleotide containing hBDNF promoter I CREB-binding sequence and lysates from KCl-treated primary rat neurons (Pruunsild, P., et al. (2011). J. Neurosci. 31(9):3295-3308).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

This antibody recognizes CREB.

Protein A purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CREB in 10 µg of HeLa nuclear extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-218

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