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Merck

1.12018

Albumin fraction V

(from bovine serum) for biochemistry

동의어(들):

Albumin fraction V, Bovine serum albumin, Serum albumin

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
MDL number:
NACRES:
NA.21
EC Index Number:
292-322-5
UNSPSC Code:
12352202
Biological source:
bovine
Form:
solid
Technique(s):
immunoassay: suitable, western blot: suitable
Concentration:
≥97% protein (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))
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biological source

bovine

form

solid

concentration

≥97% protein (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))

technique(s)

immunoassay: suitable, western blot: suitable

pH

6.8-7.2 (20 °C, 1 g/L in H2O)

solubility

1 g/L

bulk density

150 kg/m3

storage temp.

2-8°C

General description

Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each. This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.

Application

Albumin fraction V has been used:
  • as a component of perfusion buffer for perfusion protocol for rodent organ perfusion
  • as a blocking agent in western blot analysis
  • as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the chief component of cell culture media and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA). It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids. 

Analysis Note

Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Sebastian Ivens et al.
Brain : a journal of neurology, 130(Pt 2), 535-547 (2006-11-24)
It has long been recognized that insults to the cerebral cortex, such as trauma, ischaemia or infections, may result in the development of epilepsy, one of the most common neurological disorders. Human and animal studies have suggested that perturbations in
J L Lanciego et al.
Brain research. Brain research protocols, 2(4), 323-332 (1998-06-19)
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Thomas Franze et al.
The Analyst, 129(7), 589-596 (2004-06-24)
Three monoclonal antibodies (mAb) and three polyclonal antibodies (pAb) have been characterized and compared with respect to their cross-reactivities and affinities for 3-nitrotyrosine, eight aromatic compounds with similar chemical structures, a peptide containing a single nitrotyrosine residue, and fourteen nitrated
Holger Jungbluth et al.
Clinical oral investigations, 18(9), 2077-2086 (2014-02-08)
The aim of this series of studies was the development and validation of a new model for evaluation of dentinal hypersensitivity (DH) therapies. Roots from extracted human teeth were sealed with a flowable composite. In the cervical area, a 3-mm-wide
Charlotte Bayer Christiansen et al.
American journal of physiology. Gastrointestinal and liver physiology, 315(1), G53-G65 (2018-03-02)
The colonic epithelium harbors a large number of endocrine cells, but little is known about the endocrine functions of the colon. However, the high density of glucagon like peptide-1 (GLP-1)- and peptide-YY (PYY)-secreting L cells is of great interest because

문서

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

국제 무역 품목 번호

SKUGTIN
1.12018.002504022536138350
1.12018.010004022536138367

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