Endoglycosidase F1

Name: Endoglycosidase F1
Brand: MM

Cleaves specific N-glycan structures, suitable for Mass Spectrometry, from Elizabethkingia meningosepticum, recombinant, expressed in E. coli

동의어(들):

Endo-β-N-acetylglucosaminidase F1, Endo F1

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

EC 번호:

3.2.1.96 (BRENDA, IUBMB)

UNSPSC Code:

12352202

NACRES:

NA.54

Specific activity:

≥16 units/mg protein
≥17 units/mL

Recombinant:

expressed in E. coli

주요 문서

모든 문서 보기

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제품 이름

Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli, Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins.

recombinant

expressed in E. coli

conjugate

(N-linked)

form

liquid

specific activity

≥16 units/mg protein
≥17 units/mL

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

foreign activity

Proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Quality Level

100

General description

Note: 1 mU = 1 milliunit.

Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.

Disclaimer

Toxicity: Standard Handling (A)

Other Notes

One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5.

Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

Physical form

In 20 mM Tris-HCl, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

저장 등급

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Multiple endoglycosidase (Endo) F activities expressed by Flavobacterium meningosepticum. Endo F1: molecular cloning, primary sequence, and structural relationship to Endo H.

A L Tarentino et al.

The Journal of biological chemistry, 267(6), 3868-3872 (1992-03-06)

A full-length insert for the endo-beta-N-acetylglucosaminidase (Endo) F1 gene was located on a 2,200-base pair EcoRI fragment of genomic DNA and cloned into the plasmid vector Bluescript. Transformed Escherichia coli cells expressed Endo F1 activity very well, but the enzyme

Enzymatic deglycosylation of asparagine-linked glycans: purification, properties, and specificity of oligosaccharide-cleaving enzymes from Flavobacterium meningosepticum.

A L Tarentino et al.

Methods in enzymology, 230, 44-57 (1994-01-01)

Identification of distinct endoglycosidase (endo) activities in Flavobacterium meningosepticum: endo F1, endo F2, and endo F3. Endo F1 and endo H hydrolyze only high mannose and hybrid glycans.

R B Trimble et al.

The Journal of biological chemistry, 266(3), 1646-1651 (1991-01-25)

Flavobacterium meningosepticum endo-beta-acetyl-glucosaminidase F preparations have been resolved by hydrophobic interaction chromatography on TSK-butyl resin into at least three activities designated endo F1, endo F2 and endo F3 each with a unique substrate specificity. The 32-kDa endo F1 protein is

A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans.

Thapakorn Jaroentomeechai et al.

Nature communications, 13(1), 6325-6325 (2022-10-25)

The ability to reconstitute natural glycosylation pathways or prototype entirely new ones from scratch is hampered by the limited availability of functional glycoenzymes, many of which are membrane proteins that fail to express in heterologous hosts. Here, we describe a

문서

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

국제 무역 품목 번호

SKU	GTIN
324725-700MIUCN	04055977215991

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.

Endoglycosidase F1

Cleaves specific N-glycan structures, suitable for Mass Spectrometry, from Elizabethkingia meningosepticum, recombinant, expressed in E. coli

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

recombinant

conjugate

form

specific activity

manufacturer/tradename

storage condition

foreign activity

shipped in

storage temp.

Quality Level

관련 카테고리

설명

General description

Disclaimer

Other Notes

Physical form

Legal Information

안전성 정보

저장 등급

wgk

flash_point_f

flash_point_c

문서

시험 성적서(COA)

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