70746
Benzonase® Nuclease
Purity > 90%
동의어(들):
Endonuclease from Serratia marcescens
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Assay:
>90% (SDS-PAGE)
Biological source:
Serratia marcescens
Recombinant:
expressed in E. coli
Concentration:
25-29 units/μL
제품 이름
Benzonase® Nuclease, Purity > 90%,
biological source
Serratia marcescens
recombinant
expressed in E. coli
assay
>90% (SDS-PAGE)
form
buffered aqueous glycerol solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
25-29 units/μL
application(s)
research use
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
Used for the removal of nucleic acid from protein samples.
Biochem/physiol Actions
Digests native or heat-denatured DNA and RNA.
Disclaimer
Toxicity: Standard Handling (A)
General description
Benzonase® Nuclease is a geneticallyengineered endonuclease from Serratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster andPopCulture Protein Extraction Reagentsand can therefore be added along with thesereagents to eliminate viscosity and removenucleic acids from E. coli extracts.
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >90% by SDS-PAGE
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >90% by SDS-PAGE
Other Notes
One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
Legal Information
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
10 - Combustible liquids
wgk
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.
The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 70746-4CN | 04055977256949 |
| 70746-3CN | 07790788052805 |
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