70967
GeneJuice® Transfection Reagent
Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.
동의어(들):
GeneJuice Transfection, Transfection Reagent
제품 이름
GeneJuice® Transfection Reagent, Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
transfection: suitable
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Disclaimer
Toxicity: Flammable (J)
Features and Benefits
- Highly efficient DNA transfer for both stable and transient transfections
- Compatibility with both serum-containing and serum-free media
- Simple protocolno need for media changes
- Ideal for high-throughput transfection in a multi-well plate format
- Ideal for retrovirus production for T-cell transduction
- Works for a multitude of cell types with minimal optimization
- Provides higher transfection efficiency and lower cytotoxicity than reagents from other suppliers
- The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates
General description
""I use GeneJuice because it′s ease-of-use, low toxicity and wide range of target cells""
Stuart Rulten. Research Fellow. University of Sussex
""Since we have been working with GeneJuice, we have saved a lot of time and money!""
Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany
""I find that Genejuice works for a multitude of cell types with minimal optimization""
Caitriona Marie Lyons. PhD Student. University College Cork, Ireland
Transfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.
Stuart Rulten. Research Fellow. University of Sussex
""Since we have been working with GeneJuice, we have saved a lot of time and money!""
Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany
""I find that Genejuice works for a multitude of cell types with minimal optimization""
Caitriona Marie Lyons. PhD Student. University College Cork, Ireland
Transfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.
Cell lines transfected with GeneJuice® Transfection Reagent
| Cell Lines: | Primary Cells: | ||||
|---|---|---|---|---|---|
| 10T1/2 293T 3T3 NIH 3T3 Swiss 3T3-L1 A204 A431 A549 alpha TC1-6 AR 42J As4.1 AtT-20 B50 BC-1 BC-2 BC3 BCBL BHK-21 C3H/10T1/2 C6 C2C12 | Caco-2 Caki-1 Calpan-1 Calu-1 Calu-6 CCL-131 CFPAC-1 Chang Liver CHO CHO-7 CHO-IR CHO-K1 COS-1 COS-7 CS-1 CV-1 Daudi DDTI MF-2 DT40 ECV304 EL4 | ES-E14TG2a EVSCC17M H9c2 HCT-116 HEK293 HeLa HeLa B HeLa T4 Hep 3B2.1-7 HepG2 Hepa 1-6 Ht-29 HTB-37 HTB-45 Huh-7 HUVEC IC21 IEC-6 JEG-3 Jurkat KB | L57-3-11 L-6 L-929 MA-10 McA-RH7777 MCF-7 MCF-10-2A MDCK Melanocyte MG-63 Neuro 2A Neuroblastoma NRK NT2/D1 OV-1063 OVCAR3 P4 P19 PC12 PA317 PAM212 | PS-1 R2C RAW 264.7 RBL-2H3 RMP-41 SAOS-2 SC-1 Schneider line2 SK-N-MC SK-N-SH SKOV3 STO SW-480 SW-837 T3M4 TM4 U937 UCD Vero | Aortic smooth muscle cells Astrocytes Angioblasts Chondrocytes Chromaffin cells Epithelial cells: mammary prostate tracheal Fibroblasts Keratinocytes |
Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.
Legal Information
GeneJuice® is a registered trademark of EMD Chemicals Inc.
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Preparation Note
For protocol, please click (here)
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
저장 등급
3 - Flammable liquids
wgk
WGK 1
flash_point_f
67.1 °F - Information taken from reference works and the literature.
flash_point_c
19.5 °C - Information taken from reference works and the literature.
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Linda Brunotte et al.
The Journal of biological chemistry, 286(44), 38748-38756 (2011-09-16)
The nucleoprotein (NP) of Lassa virus (LASV) strain AV was expressed in a recombinant baculovirus system. The crystal structure of full-length NP was solved at a resolution of 2.45 Å. The overall fold corresponds to that of NP of LASV
Sharon Eisenberg et al.
Molecular and cellular biology, 31(19), 3938-3952 (2011-08-03)
The trafficking, membrane localization, and lipid raft association of Ras proteins, which are crucial oncogenic mediators, dictate their isoform-specific biological responses. Accordingly, their spatiotemporal dynamics are tightly regulated. While extensively studied for H- and K-Ras, such information on N-Ras, an
Natasha C Lucki et al.
The Journal of biological chemistry, 286(22), 19399-19409 (2011-04-16)
Sphingolipid metabolites, such as ceramide (Cer), sphingosine (SPH), and sphingosine 1-phosphate (S1P), contribute to multiple aspects of carcinogenesis including cell proliferation, migration, angiogenesis, and tumor resistance. The cellular balance between Cer and S1P levels, for example, is an important determinant
Mark G Frost et al.
Bioscience reports, 40(6) (2020-05-19)
Fanconi Anemia (FA) is a rare genetic disorder characterized by developmental defects, bone marrow failure and high predisposition to cancer. The FA DNA repair pathway is required in humans to coordinate repair of DNA interstrand cross-links. The central event in
Karel De Gendt et al.
Biology of reproduction, 85(5), 934-945 (2011-07-08)
Our previous analysis of Sertoli cell androgen receptor (AR) knockout (SCARKO) mice revealed that several cytoskeletal components are a potential target of androgen action. Here, we found that one of these components, the beta-tubulin isotype Tubb3, is differentially regulated in
관련 콘텐츠
Major technological advances have made the production of monoclonal antibodies quicker and more efficient. There are three established platforms for antibody discovery. We offer reagents for production of monoclonal antibody libraries using each of these techniques.
최근 기술 분야에서 중대한 발전들이 이뤄지면서 단클론 항체 생성의 속도와 효율성이 더욱 높아졌습니다. 항체 발견을 위해 확립된 플랫폼은 3가지가 있습니다. Merck는 이 기법들을 각각 이용해서 단클론 항체 라이브러리를 생성하는 데 필요한 시약을 제공합니다.
Successful delivery of nucleic acids and protein in eukaryotic cells requires a transfection protocol that maximizes transfection efciency, minimizes cytotoxicity, and results in the desired level of gene expression or silencing. EMD Millipore recognizes that there is not a one-size-ts-all solution to your transfection needs. We provide the breadth of transfection reagents necessary to give you the freedom to design the perfect experiment.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 219407-25MGCN | 07790788048716 |
| 137910BR | 04061838962232 |
| 70967-5CN | 04055977256222 |
| 70967-3CN | 07790788052928 |
| 70967-EACN | 04055977332988 |
| 70967-6CN | 04055977255577 |
| 70967-4CN | 04055977256215 |
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