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Merck

71842

KOD Hot Start Master Mix

Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., ready-to-use solution, suitable for PCR

동의어(들):

High fidelity master mix, Hot start PCR, Hot start master mix

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.55
UNSPSC Code:
41105500

주요 문서

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기술 서비스
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도움 문의
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도움 문의

form

ready-to-use solution

usage

sufficient for 100 reactions, sufficient for 500 reactions

feature

Difficult Templates/Specialty Enzymes PCR, High Fidelity PCR, dNTPs included, hotstart

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

PCR: suitable (2X PCR Master Mix for high fidelity), PCR: suitable

input

purified DNA

shipped in

dry ice

storage temp.

−20°C

Quality Level

100

관련 카테고리

General description

KOD Hot Start Master Mix is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR, which utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultra-pure deoxynucleotides, and reaction buffer with MgSO4. The monoclonal antibodies inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures.

Application

KOD Hot Start Master Mix has been used:
  • To yield PCR product for T7 endonuclease I (T7EI) assay.
  • For PCR amplification of the DNA isolated from Chrysosporium multifidum, a fungus with antimicrobial activity.
  • In the PCR amplification of cDNA.
  • In two-step PCR for the amplification of the target sequence followed by its insertion into a vector.

Features and Benefits

  • Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
  • Amplifies genomic DNA templates up to 12 kbp
  • Amplifies plasmid and lambda DNA template up to 21 kbp
  • Eliminates mispriming and primer-dimer formation
  • Convenient ambient-temperature setup compatible with automation
  • KOD HotStart® Buffer ensures optimal PCR performance over a wide range of targets.
  • Offers high specificity, fidelity and yield during PCR amplification
  • Optimized for ease of use and data reproducibility
  • Simplifies PCR set-up and saves time
  • High consistency with minimal risk of contamination

*Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).

Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

Other Notes

  • 2 × 1.25 ml or 10 × 1.25 mL KOD Hot Start Master Mix

References:
Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem.66 (10), 2194. Fujii, S., et al. 1999. J. Mol.
One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.
Simply add KOD HotStart® Master Mix to an equal volume of sample containing DNA template and primers. The final diluted reaction contains 1U KOD HotStart® DNA Polymerase per 50 μl reaction. The smaller available size provides sufficient master mix for 100 (50 μl scale), or 250 (20 μl scale) reactions, while the larger size is adequate for 500 (50 μl scale) or 1250 (20 μl scale) reactions.

Legal Information

* Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Adam S Dingens et al.
Cell host & microbe, 21(6), 777-787 (2017-06-06)
Precisely defining how viral mutations affect HIV's sensitivity to antibodies is vital to develop and evaluate vaccines and antibody immunotherapeutics. Despite great effort, a full map of escape mutants has not been delineated for an anti-HIV antibody. We describe a
Yesenia Correa et al.
PloS one, 14(12), e0218837-e0218837 (2019-12-21)
The gut microbiota of insects is composed of a wide range of microorganisms which produce bioactive compounds that protect their host from pathogenic attack. In the present study, we isolate and identify the fungus Chrysosporium multifidum from the gut of
Michael B Doud et al.
Viruses, 8(6) (2016-06-09)
Influenza genes evolve mostly via point mutations, and so knowing the effect of every amino-acid mutation provides information about evolutionary paths available to the virus. We and others have combined high-throughput mutagenesis with deep sequencing to estimate the effects of
John A Burns et al.
Nucleic acids research, 38(22), 8178-8187 (2010-08-13)
O(6)-Methylguanine (O(6)-meG), which is produced in DNA following exposure to methylating agents, instructs human RNA polymerase II to mis-insert bases opposite the lesion during transcription. In this study, we examined the effect of O(6)-meG on transcription in human cells and
Jesse D Bloom
Molecular biology and evolution, 31(8), 1956-1978 (2014-05-27)
All modern approaches to molecular phylogenetics require a quantitative model for how genes evolve. Unfortunately, existing evolutionary models do not realistically represent the site-heterogeneous selection that governs actual sequence change. Attempts to remedy this problem have involved augmenting these models
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문서

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

핫스타트 PCR

핫스타트(Hot Start) PCR의 목적은 비특이적 증폭을 줄이고 프라이머 이량체의 형성을 방지하며 제품 수율을 높이기 위해 PCR 반응을 억제하는 것입니다.

관련 콘텐츠

KOD One™ PCR Master Mix

Efficiently amplify your samples with confidence using KOD One™ PCR Master Mix for ultra-fast PCR with one of the fastest elongation rates available for high fidelity polymerases.

KOD One™ PCR Master Mix

Efficiently amplify your samples with confidence using KOD One™ PCR Master Mix for ultra-fast PCR with one of the fastest elongation rates available for high fidelity polymerases.

TB506 KOD Hot Start Master Mix

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PCR Protocols and Guides - Simplify your gene discovery

We strive to provide up-to-date PCR protocols for your greatest experimental challenges. In this guide, we share our collective expertise on technical applications of PCR to help you achieve high fidelity gene amplification using our optimized protocols for minimal sample processing. This guide includes information on sample preparation, primer design, PCR reagent set up, and thermocycling parameters. We’ve provided detailed guidelines for product usage in technical bulletins available on product-specific pages on our website.

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SKUGTIN
71842-3CN07790788053475
71842-4CN07790788053482

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