Anti-MMP-9 Antibody, Catalytic domain

92 kDa

All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM.
MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III, and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas.

E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain)

Epitope: Catalytic domain

Anti-MMP-9 Antibody, Catalytic domain is an antibody against MMP-9 for use in IH, IP & WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Western Blot Analysis:
1:1000 dilution of a previous lot was used.

Immunoprecipitation:
1:500 dilution from a previous lot was used. MMP-9 is constitutively produced by some tumor cell lines (i.e., HT1080, HL60, and U937), but not in most quiescent cells and tissues. The phorbol ester TPA stimulates production of MMP-9 in some cell types. MMP-9 levels in culture media (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I. et al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.
Western blot: 1:2,000. Typically 98-95 kDa pro and 92-88kDa active forms are detected as well as breakdown products under fully reduced conditions. Under non-reduced conditions additional bands may be detected because of SDS-stable TIMP and other inhibitor complexes. CC069 is recommended as a positive control.

Immunohistochemistry: 1:100 dilution from a previous lot was used in frozen acetone fixed tissues.

Optimal working dilutions must be determined by end user.

Reactivity with other species has not been confirmed.

Recognizes rat MMP-9 pro and active forms. Antibody also reacts with mouse MMP-9 and human MMP-9, other species not tested. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain) as an immunogen.

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing PBS, containing 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HL60 cell lysate, U937 cell lysate Lung.

Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected MMP-9 on 10 µg of L6 lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1150

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