AB3553
Anti-Thiazide-Sensitive NaCl Cotransporter Antibody
Chemicon®, from rabbit
동의어(들):
Anti-NCC, Anti-NCCT, Anti-TSC
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
제품 이름
Anti-Thiazide-Sensitive NaCl Cotransporter Antibody, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rabbit, mouse, rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SLC12A3(6559)
Analysis Note
Control
POSITIVE CONTROL: Rat kidney.
POSITIVE CONTROL: Rat kidney.
Application
Immunoblotting: 1:2,000 using rat kidney extract. The antibody reacts with the 160-190 kDa protein (the molecular weight varies depending on conditions for protein extraction/electrophoresis). The suggested dilution and blocking buffer is 150 mM NaCl, 10 mM Tris, pH 7.4 containing 5% low fat milk and 0.04% Tween. Suggested transfer membrane is PVDF or Nylon. Suggested gel percentage is 7%. Overnight incubation with the antibody at 2-8°C is recommended.
Immunohistochemistry: 1:500-1:2,000 on rat or mouse kidney tissue. Suggested fixative is 3% paraformaldehyde for 5 minutes by vascular perfusion. Suggested blocking buffer is PBS containing 10% normal goat serum. Suggested dilution buffer is PBS containing 1% BSA. Overnight incubation with the antibody at 2-8°C is recommended.Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:500-1:2,000 on rat or mouse kidney tissue. Suggested fixative is 3% paraformaldehyde for 5 minutes by vascular perfusion. Suggested blocking buffer is PBS containing 10% normal goat serum. Suggested dilution buffer is PBS containing 1% BSA. Overnight incubation with the antibody at 2-8°C is recommended.Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
This Anti-Thiazide-Sensitive NaCl Cotransporter Antibody is validated for use in IH, WB for the detection of Thiazide-Sensitive NaCl Cotransporter.
Biochem/physiol Actions
Recognizes the renal thiazide-sensitive NaCl cotransporter (NCC). The antibody stains the apical plasma membrane and subapical membrane vesicles in renal distal convoluted tubule cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
160-190 kDa
Immunogen
Synthetic peptide from the N-terminal of human/rat/mouse NCC.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB3564P
Physical form
Affinity Purified immunoglobulin in PBS containing no preservatives.
ImmunoAffinity Purified
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Genomic-derived markers for early detection of calcineurin inhibitor immunosuppressant-mediated nephrotoxicity.
Cui, Y; Huang, Q; Auman, JT; Knight, B; Jin, X; Blanchard, KT; Chou, J; Jayadev, S; Paules, RS
Toxicological Sciences null
Renal tubule angiotensin II type 1 receptor-associated protein promotes natriuresis and inhibits salt-sensitive blood pressure elevation.
Wakui, H; Uneda, K; Tamura, K; Ohsawa, M; Azushima, K; Kobayashi, R; Ohki, K; Dejima et al.
Journal of the American Heart Association null
Michael P Hell et al.
Cancer research, 74(9), 2422-2431 (2013-12-24)
The von Hippel-Lindau (VHL) tumor suppressor protein pVHL is commonly mutated in clear cell renal cell carcinoma (ccRCC) and has been implicated in the control of multiple cellular processes that might be linked to tumor suppression, including promoting proper spindle
Smail Messaoudi et al.
Nature communications, 6, 8835-8835 (2015-12-01)
Despite its high prevalence and economic burden, the aetiology of human hypertension remains incompletely understood. Here we identify the transcription factor GATA5, as a new regulator of blood pressure (BP). GATA5 is expressed in microvascular endothelial cells and its genetic
Oleg Zaika et al.
American journal of physiology. Renal physiology, 310(4), F311-F321 (2015-12-04)
Potassium Kir4.1/5.1 channels are abundantly expressed at the basolateral membrane of principal cells in the cortical collecting duct (CCD), where they are thought to modulate transport rates by controlling transepithelial voltage. Insulin and insulin-like growth factor-1 (IGF-1) stimulate apically localized
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| AB3553 | 04053252279294 |
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.