Anti-PI3 Kinase p85 Antibody, N-SH2 domain

Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt Q63787; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the Pik3r1 gene (Gene ID 25513) in rat species. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in Pik3r1 gene are known to be associated with insulin resistance.

~85/50 kDa observed. 83.47/53.49/49.97/84.47 kDa (human isoform 1/AS53/p46/p85I; UniProt P27986), 83.39 kDa (mouse; UniProt P26450), 83.40/53.39/50.00 kDa (rat p85-alpha/p55-alpha/p50-alpha isoforms; UniProt Q63787). Uncharacterized bands may be observed in some lysate(s).

Epitope: First SH2 domain.

GST-tagged recombinant fragment corresponding to the first SH2 domain of rat PI3 kinase regulatory subunit alpha.

Detect PI3 Kinase regulatory subunit alpha using this rabbit polyclonal Anti-PI3 Kinase p85, N-SH2 domain, Cat. No. ABS233, validated for use in Immunoprecipitation and Western Blotting.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 200 µg of rat brain tissue lysate.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 500 µg of Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL from a representative lot detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of Jurkat cell lysate.

Research Category
Signaling

Research Sub Category
Developmental Signaling

This polyclonal antibody targets the first SH2 domain region common to human (isoform 1, AS53, p46, p85I), mouse, and rat (isoform p85-alpha, p55-alpha, and p50-alpha) spliced isoforms of PI3 kinase regulatory subunit alpha reported by UniProt (P27986, P26450, Q63787).

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Jurkat cell lysate

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of rat brain tissue lysate.

Concentration: Please refer to lot specific datasheet.

Replaces: 06-496

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