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ISEQ07850

Immobilon® -PSQ PVDF Membrane

50 sheets, 7 cm x 8.4 cm, 0.2 µm pore size, transfer membrane for Western blotting

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.2 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Toluidine blue, for use with Transillumination
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제품 이름

Immobilon®-PSQ PVDF Membrane, 50 sheets, 7 cm x 8.4 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Level

400

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

General description

The Immobilon®-PSQ membrane is a 0.2 μm microporous polyvinylidene fluoride (PVDF) membrane that has been developed to maximize protein binding. This transfer membrane is suitable for direct protein sequencing and immunodetection following electroblotting of protein from electrophoresis gels (or dot binding of purified protein samples). The Immobilon®-PSQ membrane offers optimal blotting for proteins with molecular weights less than 20 kDa. It has more surface area and displays higher binding capacity for small proteins.
Filter Code: ISEQ
Primary Binding Mechanisms: Electrostatic, hydrophobic

Application

Immobilon-PSQ PVDF Membrane has been used for western blotting.

Features and Benefits

Immobilon-PSQ Membrane:
  • Compatible with tank and semi-dry electroblotting systems
  • Higher protein adsorption and sequencing yields than other membranes
  • Prevents blow-through of low molecular weight proteins

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

10-13 - German Storage Class 10 to 13

시험 성적서(COA)

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관련 콘텐츠

Immobilon®-E Transfer Membrane User Guide
Western Blotting Tools

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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SKUGTIN
ISEQ0785004053252269950