MAB1998
Anti-Integrin α2β1 Antibody, clone BHA2.1
clone BHA2.1, Chemicon®, from mouse
동의어(들):
VLA-2
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
제품 이름
Anti-Integrin α2β1 Antibody, clone BHA2.1, clone BHA2.1, Chemicon®, from mouse
conjugate
unconjugated
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BHA2.1, monoclonal
species reactivity
human, canine, pig
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ITGA2(3673)
관련 카테고리
Analysis Note
Control
Skin, CD4 & CD8 positive T cells
Skin, CD4 & CD8 positive T cells
Application
Anti-Integrin α2β1 Antibody, clone BHA2.1 is an antibody against Integrin α2β1 for use in FC, IH, IH(P) & IP.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
flow cytometry: 10 μg/mL
Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1
immunohistochemistry in frozen tissue sections: 5 μg/mL
Immunohistochemistry in paraffin sections: 1:50. Block endogenous peroxidase using 0.5% H202 in 80% ethanol, 30 min and unspecific reactions using NGS 1:5 in PBS, 20min RT. Bouin′s fixative solution has been used successfully. Incubation time, conditions: 24 h; 4°C; pretreatment: 0.1% trypsin, 30 min, 37°C. Intracytoplasmic staining near cell membranes.
Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.
Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.
Final working dilutions must be determined by end user.
Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1
immunohistochemistry in frozen tissue sections: 5 μg/mL
Immunohistochemistry in paraffin sections: 1:50. Block endogenous peroxidase using 0.5% H202 in 80% ethanol, 30 min and unspecific reactions using NGS 1:5 in PBS, 20min RT. Bouin′s fixative solution has been used successfully. Incubation time, conditions: 24 h; 4°C; pretreatment: 0.1% trypsin, 30 min, 37°C. Intracytoplasmic staining near cell membranes.
Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.
Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.
Final working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with the collagen receptor VLA-2 (alpha-2beta-1) integrin complex.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein A purified
Purified immunoglobulin from Protein A chromatography. Presented in liquid in 0.02M PB pH 7.6, 0.25M NaCl, 0.1% sodium azide.
Preparation Note
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Amna Abderrazak et al.
Frontiers in immunology, 9, 2269-2269 (2018-10-31)
β1 integrins are critical for T cell migration, survival and costimulation. The integrin α2β1, which is a receptor for collagen, also named VLA-2, is a major costimulatory pathway of effector T cells and has been implicated in arthritis pathogenesis. Herein
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International journal of nanomedicine, 13, 7657-7667 (2018-12-13)
It is well known that zirconia materials have good biocompatibility; however, little is known regarding the mechanism by which cells attach to these materials. The purpose of this study is to elucidate the mechanism of cell attachment. In this study
Mehdi Dehghani et al.
International journal of cell biology, 2014, 981750-981750 (2015-01-13)
The mechanisms by which prostate cancer (PCa) cell adhesion and migration are controlled during metastasis are not well understood. Here, we studied the effect of CXCL12 in PCa cell adhesion and spreading in DU145 and PC3 cell lines using as
Xiaodong Feng et al.
International journal of cell biology, 2013, 231279-231279 (2013-06-06)
Angiogenesis is a highly regulated event involving complex, dynamic interactions between microvascular endothelial cells and extracellular matrix (ECM) proteins. Alteration of ECM composition and architecture is a hallmark feature of wound clot and tumor stroma. We previously reported that during
Adrian P Rybak et al.
Biochimica et biophysica acta, 1813(5), 683-694 (2011-02-01)
While accumulating evidence demonstrates the existence of prostate cancer stem cells (PCSCs), PCSCs have not been isolated and thoroughly characterized. We report here the enrichment and characterization of sphere-propagating cells with stem-like properties from DU145 PC cells in a defined
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| MAB1998 | 04053252464294 |
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