Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9

Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.

~100-110 kDa

Epitope: C-terminus

MBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1.

Anti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Works poorly for immunohistochemistry and immunocytochemistry.
Not recommended for immunoprecipitation.

Immunoblotting:
1:1,000 dilution used on a previous lot.

Optimal working dilutions must be determined by the user.

Na+/H+ exchanger, isoform NHE1

Protein A purified

Format: Purified

Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide

Stable for 1 year at 2-8ºC from date of receipt.

Control
Kidney tissue (basolateral membrane)

Routinely evaluated by Western Blot on Human Kidney lysates.

Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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