MAB3446
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9
ascites fluid, clone 2HP-2G9, Chemicon®
동의어(들):
HP1a, Antigen p25, Chromobox Protein Homolog 5
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2HP-2G9, monoclonal
Application:
ELISA, IP, WB
Citations:
20
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2HP-2G9, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CBX5(23468)
General description
25 kDa
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Immunogen
Recombinant mouse HP1alpha.
Application
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.
ELISA: 1:500-1:5,000
Western blot: 1:500-1:5,000
Immunoprecipitation: 1:500-1:5,000
Immunohistochemistry/Immunocytochemistry: Not Recommended
Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.
Optimal working dilutions must be determined by the end user.
Western blot: 1:500-1:5,000
Immunoprecipitation: 1:500-1:5,000
Immunohistochemistry/Immunocytochemistry: Not Recommended
Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.
Physical form
Liquid ascites fluid containing no preservative.
Analysis Note
Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A role for heterochromatin protein 1? at human telomeres.
Canudas, S; Houghtaling, BR; Bhanot, M; Sasa, G; Savage, SA; Bertuch, AA; Smith, S
Genes & Development null
Aurora-B/AIM-1 regulates the dynamic behavior of HP1alpha at the G2-M transition.
Terada, Y
Molecular Biology of the Cell null
Xiaoyuan Wei et al.
Journal of molecular cell biology, 6(5), 409-420 (2014-05-20)
The revolutionary induced pluripotent stem cell (iPSC) technology provides a new means for cell replacement therapies and drug screening. Small molecule compounds have been found extremely useful to improve the generation of iPSCs and understand the reprogramming mechanism. Here we
Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons.
Rangasamy, D
Mobile DNA null
Andrey Poleshko et al.
Journal of virology, 82(5), 2313-2323 (2007-12-21)
Integrated retroviral DNA is subject to epigenetic gene silencing, resulting in loss of expression of viral genes as well as reporter or therapeutic genes transduced by retroviral vectors. Possible mediators of such silencing include the histone deacetylase (HDAC) family of
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| MAB3446 | 04053252579042 |
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