MABE146
Anti-5-methylcytosine Antibody, clone 33D3
clone 33D3, from mouse
동의어(들):
methylcytosine, methylcytidine
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
33D3, monoclonal
Species reactivity:
human, mouse
Application:
Southern blotting
dot blot
flow cytometry
immunofluorescence
immunohistochemistry
methylated DNA immunoprecipitation (MeDIP)
dot blot
flow cytometry
immunofluorescence
immunohistochemistry
methylated DNA immunoprecipitation (MeDIP)
Technique(s):
Southern blotting: suitable
dot blot: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
methylated DNA immunoprecipitation (MeDIP): suitable
dot blot: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
methylated DNA immunoprecipitation (MeDIP): suitable
Citations:
48
제품 이름
Anti-5-methylcytosine Antibody, clone 33D3, clone 33D3, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
33D3, monoclonal
species reactivity
human, mouse
technique(s)
Southern blotting: suitable
dot blot: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
methylated DNA immunoprecipitation (MeDIP): suitable
isotype
IgG1λ
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
관련 카테고리
General description
5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.
Detects methylated cytosine
Immunogen
Ovalbumin-conjugated 5-methylcytosine
Other Notes
Replaces: MABE527
Physical form
Format: Purified
Protein A
Purified mouse monoclonal IgG1λ in buffer containing PBS and 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Methylated and unmethylated BRCA1
Methylated and unmethylated BRCA1
Evaluated by Dot Blot in methylated and unmethylated BRCA1.
Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.
Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.
Application
IF
Habib, M., et al. (1999). Exp Cell Res. 249:46-53
IHC-P
Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693
Flow Cytometry
Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262
MeDIP
Keshet, I., et al. (2006). Nat Genet. 38(2):149-153
Habib, M., et al. (1999). Exp Cell Res. 249:46-53
IHC-P
Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693
Flow Cytometry
Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262
MeDIP
Keshet, I., et al. (2006). Nat Genet. 38(2):149-153
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Use Anti-5-methylcytosine Antibody, clone 33D3 (mouse monoclonal antibody) validated in DB, IF, SB, IHC(P), Flow Cytometry, MeDIP to detect 5-methylcytosine also known as methylcytosine, methylcytidine.
Biochem/physiol Actions
Predicted to react with multiple species.
This antibody detects 5-Methylcytosine, but not unmethylated cytosine.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Elena M Pugacheva et al.
Epigenetics & chromatin, 9(1), 35-35 (2016-09-03)
A common aberration in cancer is the activation of germline-specific proteins. The DNA-binding proteins among them could generate novel chromatin states, not found in normal cells. The germline-specific transcription factor BORIS/CTCFL, a paralog of chromatin architecture protein CTCF, is often
Shaohai Fang et al.
Nature communications, 10(1), 4297-4297 (2019-09-22)
Tet-mediated DNA demethylation plays an important role in shaping the epigenetic landscape and chromatin accessibility to control gene expression. While several studies demonstrated pivotal roles of Tet in regulating embryonic development, little is known about their functions in heart development.
Yize Zhang et al.
Scientific reports, 7, 43158-43158 (2017-02-23)
In vertebrates, DNA methyltransferase 3 (Dnmt3) homologues are responsible for de novo DNA methylation and play important roles in germ cell development. In the present study, four dnmt3 genes, dnmt3aa, dnmt3ab, dnmt3ba and dnmt3bb.1, were identified in ricefield eels. Real-time
Alain Cuna et al.
American journal of respiratory cell and molecular biology, 53(1), 60-73 (2014-11-12)
DNA methylation, a major epigenetic mechanism, may regulate coordinated expression of multiple genes at specific time points during alveolar septation in lung development. The objective of this study was to identify genes regulated by methylation during normal septation in mice
Annabelle Congras et al.
Biology of reproduction, 91(6), 137-137 (2014-10-17)
Male infertility is an increasing health issue in today's society for both human and livestock populations. In livestock, male infertility slows the improvement of animal selection programs and agricultural productivity. There is increasing evidence that epigenetic marks play an important
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국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| MABE146 | 04053252558344 |
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