NE1022
PhosphoDetect Anti-Neurofilament H Mouse mAb (SMI-31)
liquid, clone SMI-31, Calbiochem®
동의어(들):
Anti-neurofilament antibody
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
SMI-31, monoclonal
Species reactivity:
mouse, human, rat
Application:
—
Citations:
27
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
SMI-31, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, rat
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (1:1000)
Frozen Sections (1:1000)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000)
Paraffin Sections (1:1000, heat pre-treatment required)
Immunoprecipitation )
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NEFH(4744)
General description
Mouse monoclonal antibody supplied as purified antibody. Recognizes the ~180-200 kDa phosphorylated neurofilament H protein.
Recognizes the ~180-200 kDa phosphorylated neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations. Also weakly recognizes phosphorylated NF-M protein.
This PhosphoDetect Anti-Neurofilament H Mouse mAb (SMI-31) is validated for use in ELISA, Frozen Sections, Immunoblotting, ICC, Paraffin Sections, IP for the detection of Neurofilament H.
Immunogen
homogenized, hypothalami from Fischer 344 rat brain
Application
ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Immunoprecipitation (see comments)
Physical form
Phosphate buffer solution, pH7.2, containing 0.09% sodium azide
Other Notes
Raina, A.K., et al. 1999. Neuroreport10, 1355.
Yang, C.C., et al. 1998. Brain121, 1089.
Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem.271, 30404.
Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol.55, 774.
Xiao, J. and Monteiro, M.J. 1994. J. Neurosci.14, 1820.
Yang, C.C., et al. 1998. Brain121, 1089.
Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem.271, 30404.
Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol.55, 774.
Xiao, J. and Monteiro, M.J. 1994. J. Neurosci.14, 1820.
Strongly recognizes phosphorylated neurofilament H and, to a lesser extent, phosphorylated neurofilament M. By immunocytochemistry this antibody broadly stains thick and thin axons and some dendrites, such as basket cell dendrites, but not Purkinje cell dendrites. Does not generally stain nerve cell bodies or other cells and tissues except peripheral axons. May also stain neuronal cell bodies in pathological conditions. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures following treatment with agents that induce stress-activated protein kinase. This antibody is reported to co-immunoprecipitate neurofilament-associated kinase (NAK 115) via interaction of the antibody with the tail domain of neurofilament H. Phosphatase treatment of tissue sections or immunoblotting samples abolishes antibody reactivity. Reactivity is unaffected by trypsin treatment of samples. Tissues and cultured cells can be fixed with a variety of paraformaldehyde- or formaldehyde-containing fixatives, including Bouin′s fixative. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the epitope in frozen sections or thick tissue sections fixed in 4% paraformaldehyde and in cultured cells. For staining formalin-fixed, paraffin sections it is recommended that the de-paraffinized tissue be autoclaved in dH2O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Takuya Hayashi et al.
Molecular neurodegeneration, 8, 4-4 (2013-01-22)
Neurodegenerative diseases including Parkinson's and Alzheimer's diseases progress slowly and steadily over years or decades. They show significant between-subject variation in progress and clinical symptoms, which makes it difficult to predict the course of long-term disease progression with or without
Seungyong Lee et al.
Nature communications, 12(1), 4939-4939 (2021-08-18)
Pain is a central feature of soft tissue trauma, which under certain contexts, results in aberrant osteochondral differentiation of tissue-specific stem cells. Here, the role of sensory nerve fibers in this abnormal cell fate decision is investigated using a severe
Michael J Whitehead et al.
Scientific reports, 8(1), 5219-5219 (2018-03-28)
Axon degeneration underlies many nervous system diseases; therefore understanding the regulatory signalling pathways is fundamental to identifying potential therapeutics. Previously, we demonstrated heparan sulphates (HS) as a potentially new target for promoting CNS repair. HS modulate cell signalling by both
Justin C Burrell et al.
Neurosurgery, 87(4), 833-846 (2020-05-12)
Millions of Americans experience residual deficits from traumatic peripheral nerve injury (PNI). Despite advancements in surgical technique, repair typically results in poor functional outcomes due to prolonged periods of denervation resulting from long regenerative distances coupled with slow rates of
Justin C Burrell et al.
Journal of biomedical materials research. Part A, 109(7), 1183-1195 (2020-09-29)
Promising biomaterials should be tested in appropriate large animal models that recapitulate human inflammatory and regenerative responses. Previous studies have shown tyrosine-derived polycarbonates (TyrPC) are versatile biomaterials with a wide range of applications across multiple disciplines. The library of TyrPC
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| NE1022-100ULCN | 04055977209907 |
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