CT-2A-Luc Mouse Glioma Cell Line

Glioblastomas are among the most aggressive forms of cancer, associated with low treatment efficacy and poor survival. Recurring glioblastomas are often resistant to first-line chemotherapies (1). There is much interest in studying drug-resistant forms of glioblastomas in the effort to develop effective therapies.

References:
1. Weller M, Cloughesy T, Perry JR, and Wick W (2013) Neuro Oncol 15(1): 4-27.
2. Seyfried TN, Mukherjee P (2010) J Oncol 2010:961243 doi.10.1155/2010/961243.
3. Cotterchio M, Seyfried TN (1994) J Lipid Res 35(1): 10-14.
4. Binello E, Qadeer ZA, Kothari HP, Emdad L, Germano IM (2012) J Cancer 3: 166-174.
5. Martinez-Murillo R, Martinez A (2007) Histol Histopathol 22(12): 1309-1326.
6. Zimmerman HM and Arnold H. (1941) Cancer Res 1(12): 919-938.
7. Huysentruyt LC, Mukherjee P, Banerjee D, Shelton LM, Seyfried TN (2008) Int J Cancer 123(1): 73-84.

The CT-2A-Luc cell line is derived from a sub-cutaneous, non-metastatic murine glioma (astrocytoma) and is stably transduced with a firefly luciferase-EGFP reporter. CT-2A-Luc cells are marked by high levels of complex gangliosides and low distribution of the anti-angiogenic ganglioside GM3, as well as deficiency in the tumor suppressor PTEN/TSC2, a characteristic present in up to 70% of human high-grade glioma cell lines (2,3). CT-2A tumors are wild-type for p53 and recapitulate several features of human high-grade glioma, including high mitotic index and cell density, nuclear polymorphism, hemorrhage, pseudopalisading necrosis, and microvascular proliferation (4,5).

Source:
The parental CT-2A cell line was generated from a malignant astrocytoma formed via implantation of the carcinogen 20-methylcholanthrene in the cerebrum of a C57BL/6J mouse (6). The tumor was maintained through serial intracranial transplants prior to cell line isolation. The CT-2A-Luc luciferase cell line was clonally derived from CT-2A cells transduced with a lentiviral vector harboring a firefly luciferase (Fluc)-IRES-GFP cassette under control of the CMV promoter; cells were subsequently sorted for EGFP expression (7).

Research Category
Cancer

Oncology

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Cancer Cells

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

• Each vial contains 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination