Immobilon^® Block - PO (Phosphoprotein Blocker)

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

이해합니다. 서양인들은 얼룩으로 인해 고통스러울 수 있으며, 저희도 확실히 그 고통을 겪었습니다. 그러니 여러분의 못생긴 오점을 공유해 주시고 깜짝 선물을 받아보세요! 깜짝 선물이 무엇인지 알려드릴 수는 없지만, 실험실에서 창의력을 자극할 수 있다는 점만 말씀드릴 수 있습니다.

In Western blotting, blocking of unbound membrane sites is necessary to prevent non-specific binding of the antibodies which otherwise would lead to a high background on the blot. The traditional milk blocker can work well; however, inadvertent variations in its composition can lead to irreproducible results. Such variations include the milk’s concentration, fat content, solubility, detergent quality, and numerous other factors. Bløk reagents are a family of protein-free, noisecancelling reagents that reduce background for consistent, quality results. They are available in three room temperature-stable, ready-to-use formulations for Chemiluminescence and chromogenic detection, Fluorescence detection, and Phosphoprotein immunodetection. The protein-free nature of Bløk reagents allows for membranes to be stained with chromogenic reagents, such as Ponceau S or Coomassie™ blue, after blocking or immunodetection. Bløk reagents have been tested and validated for Western blot, dot blot, and ELISA applications.