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10843555001

Roche

Hygromycin B

Name: Hygromycin B
Brand: ROCHE

from Streptomyces hygroscopicus

동의어(들):

Hygromycin B from Streptomyces hygroscopicus

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):

C₂₀H₃₇N₃O₁₃

CAS 번호:

31282-04-9

Molecular Weight:

527.52

UNSPSC Code:

12352200

PubChem Substance ID:

329749191

MDL number:

MFCD06795479

NACRES:

NA.54

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도움 문의

속성

biological source

Streptomyces hygroscopicus

sterility

non-sterile; 0.2 μm filtered

assay

80% (HPLC)

form

buffered aqueous solution

composition

Hygromycin B, >80% HPLC

packaging

pkg of 20 mL (1 g)

manufacturer/tradename

Roche

concentration

50 mg/mL

technique(s)

transfection: suitable

SMILES string

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

설명

General description

Formula: C₂₀H₃₇N₃O₁₃
LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
Molecular weight: Mr = 527.5
Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

Application

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).

Biochem/physiol Actions

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Physical form

Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane

Preparation Note

Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.

Store at 2 to 8 °C. (solution)