biological source
sheep
conjugate
fluorescein conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
packaging
pkg of 200 μg
manufacturer/tradename
Roche
isotype
IgG
storage temp.
2-8°C
Quality Level
General description
Anti-Digoxigenin-Fluorescein, Fab fragments has fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to fluorescein.
Application
Anti-Digoxigenin-Fluorescein, Fab fragments are used for the detection of digoxigenin-labeled compounds using:
- Digoxigenin-labeled sugars in glycoconjugate research
- Fluorescent in situ hybridization (FISH)
- Immunohistocytochemistry
- In situ hybridization
Biochem/physiol Actions
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Preparation Note
- Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:Detection of digoxigenin-labeled sugars in glycoproteins: 50 to 20μg/ml
- Fluorescent in situ hybridization (FISH): 1 to 20μg/ml
- Immunohistocytochemistry: 20 to 50μg/ml
- In situ hybridization: 20 to 50μg/ml
The anti-DIG anitbody is highly specific and sensitive. Therefore, for FISH, a concentration of 1μg/ml is sufficient, and only in case of critical probes, this concentration may be raised to <10μg/ml (with a distinct background to be expected).
Working solution: Reconstitution
Add 1 ml double-distilled water to a final concentration of 200μg/ml.
Blocking:
PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore, pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
Add 1 ml double-distilled water to a final concentration of 200 μg/ml.
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
저장 등급
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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