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제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352202
NACRES:
NA.54
Form:
liquid
Biological source:
Streptomyces sp. ( Streptomyces avidinii)
Recombinant:
expressed in E. coli
Mol wt:
~52 kDa
biological source
Streptomyces sp. ( Streptomyces avidinii)
recombinant
expressed in E. coli
form
liquid
specific activity
≥17 units/mg protein
mol wt
~52 kDa
packaging
pkg of 1 mg (11721666001), pkg of 5 mg (11721674001)
manufacturer/tradename
Roche
concentration
70-90%
technique(s)
protein array: suitable, protein purification: suitable
impurities
6-7% water
pH range
5.0-9.0
pI
7.4-7.7 (two main bands)
solubility
water: 10 mg/L (mA405=100)
suitability
suitable for immunological tests
UniProt accession no.
shipped in
cooler
storage temp.
-10 to -25°C
관련 카테고리
General description
Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.
Application
Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).
Physical form
White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1)
KD: 10-15M
Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1)
KD: 10-15M
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit binds 1 μg D-biotin at pH 8.9.
Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Production of Recombinant Streptavidin and Optimization of Refolding Conditions for Recovery of Biological Activity
Jouybari RM et al.
Reports of Biochemistry and Molecular Biology, 6(2), 178-185 (2018)
Automated combinatorial process for nanofabrication of structures using bioderivatized nanoparticles.
Dehlinger D, et al.
Journal of the Association for Laboratory Automation, 5, 267-276 (2007)
Michael Weber et al.
Nature photonics, 15(5), 361-366 (2021-05-07)
We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point
John E G McCarthy et al.
Methods in enzymology, 430, 247-264 (2007-10-05)
A growing number of biophysical techniques use immobilized reactants for the quantitative study of macromolecular reactions. Examples of such approaches include surface plasmon resonance, atomic force microscopy, total reflection fluorescence microscopy, and others. Some of these methods have already been
Michael Weber et al.
Nature biotechnology, 41(4), 569-576 (2022-11-08)
Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the
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