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XTGHP-RO

Roche

X-tremeGENE^™ HP DNA Transfection Reagent

Name: X-tremeGENE<SUP>™</SUP> HP DNA Transfection Reagent
Brand: ROCHE

High-performance polymer reagent for transfecting many cell lines

동의어(들):

transfection reagent

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보기 변경

제품정보 (DICE 배송 시 비용 별도)

NACRES:

NA.55

UNSPSC Code:

41106502

기술 서비스

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도움 문의

속성

grade

Molecular Biology

form

liquid (aqueous solution)

usage

mL (suitable for 165 transfections)

packaging

pkg of 0.4 mL (06366244001), pkg of 1.0 mL (06366236001), pkg of 5 × 1 mL (06366546001)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

storage temp.

−20°C

설명

General description

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Features and Benefits

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
Generate physiologically relevant data using a reagent with low cytotoxic effects.
Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Analysis Note

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

pictograms

GHS02,GHS07

signalword

Danger

pcodes

P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

저장 등급

3 - Flammable liquids

wgk

WGK 1

flash_point_f

334.4 °F

flash_point_c

168 °C

hcodes

H225,H319

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시험 성적서(COA)

Lot/Batch Number

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프로토콜

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

모든 프로토콜 보기

문서

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

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