114391
Bromophenol Blue
ACS reagent
동의어(들):
3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C19H10Br4O5S
CAS 번호:
Molecular Weight:
669.96
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
204-086-2
Beilstein/REAXYS Number:
61698
MDL number:
InChI key
UDSAIICHUKSCKT-UHFFFAOYSA-N
InChI
1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
SMILES string
Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
grade
ACS reagent
form
powder
technique(s)
titration: suitable
visual transition interval
3.0-4.6, yellow to blue
mp
273 °C (lit.)
solubility
ethanol: 0.1%
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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General description
Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.
저장 등급
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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