221198
Formamide
ACS reagent, ≥99.5%
동의어(들):
Amide C1, Formic amide
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
HCONH2
CAS 번호:
Molecular Weight:
45.04
EC Number:
200-842-0
UNSPSC Code:
12352111
PubChem Substance ID:
Beilstein/REAXYS Number:
505995
MDL number:
Assay:
≥99.5%
Grade:
ACS reagent
Technique(s):
PCR: suitable
Bp:
210 °C (lit.)
Vapor pressure:
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
InChI key
ZHNUHDYFZUAESO-UHFFFAOYSA-N
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
SMILES string
NC=O
grade
ACS reagent
vapor density
1.55 (vs air)
vapor pressure
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
assay
≥99.5%
form
liquid
autoignition temp.
932 °F
technique(s)
PCR: suitable
color
APHA: ≤10
Quality Level
pH
4-10 (20 °C, 200 g/L)
bp
210 °C (lit.)
mp
2-3 °C (lit.)
transition temp
freezing point 2.0-3.0 °C
density
1.134 g/mL at 25 °C (lit.)
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General description
Our premium ACS solvents are ideal for routine chemical synthesis, drying, purification, and critical labware cleaning. They meet or exceed the rigorous standards of the American Chemical Society (ACS), ensuring high-quality results for your research needs.
Premium ACS Solvents: Our solvents meet or exceed the stringent standards set by the American Chemical Society, ensuring high quality and reliability for your laboratory applications.
Replicable and Publishable Results: Designed for consistency, our solvents deliver results that can be reliably reproduced, making them ideal for research that requires publication.
Versatile Applications: Suitable for routine chemical synthesis, drying, purification, and critical labware cleaning, our solvents cater to a wide range of research needs in the laboratory.
Premium ACS Solvents: Our solvents meet or exceed the stringent standards set by the American Chemical Society, ensuring high quality and reliability for your laboratory applications.
Replicable and Publishable Results: Designed for consistency, our solvents deliver results that can be reliably reproduced, making them ideal for research that requires publication.
Versatile Applications: Suitable for routine chemical synthesis, drying, purification, and critical labware cleaning, our solvents cater to a wide range of research needs in the laboratory.
Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
The addition of formamide may enhance the specificity and efficiency of amplification of PCR (polymerase chain reaction). It may be used as an ammonia equivalent for the palladium-catalyzed aminocarbonylation of 1-bromo-3-trifluoromethylbenzene to form 3-(trifluoromethyl)benzamide.
signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
target_organs
Blood
저장 등급
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
305.6 °F
flash_point_c
152 °C
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Formamide can dramatically improve the specificity of PCR.
Sarkar G, et al.
Nucleic Acids Research, 18(24), 7465-7465 (1990)
Weinreb SM.
Science of Synthesis: Houben-Weyl Methods of Molecular Transformations, 21, 126-126 (2014)
Solubilization in formamide protects RNA from degradation.
Chomczynski P.
Nucleic Acids Research, 20(14), 3791-3791 (1992)
Microwave spectrum and molecular structure of formamide.
Costain CC and Dowling JM.
J. Chem. Phys. , 32(1), 158-165 (1960)
J Casey et al.
Nucleic acids research, 4(5), 1539-1552 (1977-01-01)
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is
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