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Merck

69889

MES monohydrate

BioUltra, Molecular Biology, ≥99.5% (T)

동의어(들):

2-Morpholinoethanesulfonic acid hydrate,, 2-morpholin-4-ylethanesulfonic acid;hydrate, 2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid monohydrate

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
C6H13NO4S · H2O
CAS 번호:
145224-94-8
Molecular Weight:
213.25
NACRES:
NA.25
PubChem Substance ID:
329762028
eCl@ss:
32129211
UNSPSC Code:
12161700
EC Number:
224-632-3
MDL number:
MFCD00149409
Beilstein/REAXYS Number:
6350956

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InChI key

MIIIXQJBDGSIKL-UHFFFAOYSA-N

InChI

1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2

SMILES string

O.OS(=O)(=O)CCN1CCOCC1

grade

Molecular Biology

product line

BioUltra

assay

≥99.5% (T)

form

powder or crystals

impurities

DNases, none detected, Insoluble matter, passes filter test, Phosphatases, none detected, Proteases, none detected, RNases, none detected

ign. residue (900 °C)

≤0.05% (as SO4)

pH

2.5-4.0 (25 °C, 0.5 M in H2O)

useful pH range

5.5-6.7

pKa (25 °C)

6.1

mp

>300 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

10.66 g/mL

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤20 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.025, λ: 280 nm Amax: 0.020

suitability

suitable for DNase I test, suitable for Western blot, suitable for molecular biology

application(s)

clinical research
diagnostic assay manufacturing
life science and biopharma

foreign activity

DNase, none detected, NICKase, none detected, RNase, none detected, protease, none detected

Quality Level

200

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관련 카테고리

General description

MES Buffer, known as one of the "Good′s" buffers designed for biological applications, plays a pivotal role in various aspects of research. This zwitterionic, morpholinic buffer is particularly valuable for its buffering capabilities within a pH range of 5.5 to 6.7. MES monohydrate, finds extensive utility in maintaining a stable environment in solutions used for cell culture media, protein-based buffer formulations, and electrophoresis running buffers. Its significance extends to the purification processes of antibodies, peptides, proteins, blood components, and growth factors. Additionally, MES stands out as an excellent choice for capillary electrochromatography, offering low ionic mobility. It serves as a safer alternative to toxic agents like cacodylate and non-zwitterionic buffers such as citrate and malate. While MES excels in buffering, it′s important to note that it is not recommended for use at pH 7.4, and alternative buffers should be explored in such cases. MES Monohydrate is also used to fine-tune the pH of growth media, stabilize enzymatic solutions, and as a component of PAGE running buffer in various experimental settings.

Application

MES Monohydrate has been used:
  • In the preparation of a solution used for the suspension and dilution of protoplasts to measure the density and number of protoplasts using a haemocytometer
  • As a component of Murashige and Skoog basal salt media
  • In the preparation of total ionic strength adjustment buffer (TISAB)
  • as a constituent in mobile phase solutions employed for the analysis of high molecular weight species and charge-related variants of biopharmaceutical proteins through SEC, HIC, and IEX chromatographic techniques

Features and Benefits

  • Suitable as a Buffer component, for Electrophoresis and Molecular Biology
  • Effective Buffering from pH 2.5-4.0 (25 °C, 0.5 M in H2O) with a pKa of 6.1 (25 °C)
  • Free from DNase, NICKase, RNase, and Protease

Preparation Note

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Other Notes

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8C for months.
Sterilization: Sterilization should be by filteration through 0.2 uM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.
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저장 등급

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Gloves

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시험 성적서(COA)

Lot/Batch Number
BCCJ1067
BCCH3217
BCCH9099
BCCF9189
BCCF9190

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