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Merck

B8522

Brilliant Blue G solution

Concentrate

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
기술 서비스
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InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI key

RWVGQQGBQSJDQV-UHFFFAOYSA-M

form

liquid

technique(s)

microbe id | staining: suitable

color

dark blue

suitability

suitable for (Suitable for for staining protein in polyacrylamide and agarose gels)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

General description

Brilliant Blue G is a methanol based stain which is especially designed for staining protein in polyacrylamide gels, but will also stain protein in agarose gels (IEF). The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Water is added directly to the bottle to produce 1 liter of ready-to-use staining solution.

Application

Brilliant Blue G solution has been used for protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It is also used for the protein staining of cells on microscopic slide.

Preparation Note

After dilution with deionized water to a final volume of one liter, solution will contain 0.1% (w/v) Brilliant Blue G, 25% (v/v) methanol and 5% (v/v) acetic acid.
One bottle equals one liter working solution. 300 ml of brilliant blue G solution concentrate is supplied in the bottle.

Analysis Note

This product has been tested for suitability on SDS-PAGE.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1

target_organs

Eyes,Central nervous system

저장 등급

3 - Flammable liquids

wgk

WGK 2

flash_point_f

49.5 °F - closed cup

flash_point_c

9.7 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
Hyung-Keun Ku et al.
Analytical biochemistry, 434(1), 178-180 (2012-12-04)
The Bradford assay is a simple method for protein quantitation, but variation in the results between proteins is a matter of concern. In this study, we compared and normalized quantitative values from two models for protein quantitation, where the residues

문서

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

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