C1484
7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin
≥95% purity (HPLC), solid
동의어(들):
1H-Pyrrole-2,5-dione, 1-(4-(7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl)phenyl)-, CPM
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C24H22N2O4
CAS 번호:
Molecular Weight:
402.44
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
MDL number:
제품 이름
7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin, ≥95% (HPLC), solid
Quality Level
assay
≥95% (HPLC)
form
solid
technique(s)
titration: suitable
color
yellow
solubility
DMSO: soluble, methanol: soluble
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
CCN(CC)c1ccc2C(C)=C(C(=O)Oc2c1)c3ccc(cc3)N4C(=O)C=CC4=O
InChI
1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
InChI key
YGIABALXNBVHBX-UHFFFAOYSA-N
General description
7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM), is a highly fluorescent, sulfhydryl (-SH) group containing coumarin derivative. It has excitation an emission wavelengths of 355 and 460 nm, respectively.
Application
7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin has been used:
- as a nontoxic substitute for conjugation with antibody(93)
- to aid fluorescent detection of Coenzyme A (CoA-SH) in human N-myristoyltransferases assay (94)
- in thermostability shift assay of recombinant protein(95)
Biochem/physiol Actions
Used to monitor release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Targeting Acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) with small molecule inhibitors for the treatment of metabolic diseases
Cao JS, et al.
The Journal of biological chemistry, 286(48), 41838-41851 (2011)
W Feng et al.
Molecular pharmacology, 55(5), 821-831 (1999-04-30)
Quinones undergo redox cycling and/or arylation reactions with key biomolecules involved with cellular Ca2+ regulation. The present study utilizes nanomolar quantities of the fluorogenic maleimide 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM) to measure the reactivity of hyperreactive sulfhydryl moieties on sarcoplasmic reticulum (SR) membranes
F C Ayers et al.
Analytical biochemistry, 154(1), 186-193 (1986-04-01)
A microfluorometric assay for thiols has been developed using the thiol-specific fluorochrome N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM). The technique may be used to quantitate either cellular or plasma thiols over a range of 0.01 to 3.0 nmol and may be used with as