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Merck

D5545

DL-Dithiothreitol

BioXtra, ≥99.0% (titration)

동의어(들):

threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
HSCH2CH(OH)CH(OH)CH2SH
CAS 번호:
3483-12-3
Molecular Weight:
154.25
UNSPSC Code:
12352201
NACRES:
NA.21
PubChem Substance ID:
24893941
EC Number:
222-468-7
Beilstein/REAXYS Number:
1719757
MDL number:
MFCD00004877

주요 문서

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InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

SMILES string

O[C@H](CS)[C@H](O)CS

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

product line

BioXtra

assay

≥99.0% (titration)

form

powder

reaction suitability

reagent type: reductant

impurities

Insoluble matter, passes filter test, ≤0.3% 4,5-dihydroxy-1,2-dithiane

pH

4.0-6.0 (20-25 °C, 0.1 m in H2O)

mp

41-44 °C (lit.)

solubility

H2O: 0.1 M, clear, colorless

anion traces

sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%, As: ≤0.0001%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.001%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Na: ≤0.1%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

absorption

≤0.100 at 280 in H2O at 0.1 M, ≤0.400 at 260 in H2O at 0.1 M

storage temp.

2-8°C

Quality Level

200

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관련 카테고리

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

저장 등급

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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시험 성적서(COA)

Lot/Batch Number
0000533990
0000525603
0000525604
0000525604
0000533990

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