D8001
3,3′-Diaminobenzidine
97% (HPLC)
동의어(들):
3,3′,4,4′-Biphenyltetramine, 3,3′,4,4′-Tetraaminobiphenyl, DAB
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크기 선택
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제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
(NH2)2C6H3C6H3(NH2)2
CAS 번호:
Molecular Weight:
214.27
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
202-110-6
Beilstein/REAXYS Number:
1212988
MDL number:
Assay:
97% (HPLC)
Form:
powder
Quality Level
assay
97% (HPLC)
form
powder
mp
175-177 °C (lit.)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
NC1=C(N)C=CC(C2=CC(N)=C(N)C=C2)=C1
InChI
1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
InChI key
HSTOKWSFWGCZMH-UHFFFAOYSA-N
Application
3,3′-Diaminobenzidine has been used as a chromogen for the visualization in immunohistochemistry of chondrosarcoma sections, mammary carcinomas and brain tissue sections.
Peroxidase substrate; reagent for spectrophotometric determination of selenium.
Biochem/physiol Actions
DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
저장 등급
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
A colorimetric method for measurement of the (peroxidase-mediated) oxidation of 3,3'-diaminobenzidine.
Fahimi HD and Herzog V
The Journal of Histochemistry and Cytochemistry, 21, 499-502 (1973)
Pathogenesis of a Thai strain of white spot syndrome virus (WSSV) in juvenile, specific pathogen-free Litopenaeus vannamei.
Escobedo-Bonilla CM, et al.
Diseases of Aquatic Organisms, 74, 85-94 (2007)
Detection of T lymphocytes in intestine of broiler chicks treated with Lactobacillus spp. and challenged with Salmonella enterica serovar Enteritidis.
Noujaim JC, et al.
Poultry Science, 87, 927-933 (2008)