I6758
IPTG
≥99% (TLC), ≤0.1% Dioxane
동의어(들):
Isopropyl β-D-1-thiogalactopyranoside, IPTG, Isopropyl β-D-thiogalactoside
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C9H18O5S
CAS 번호:
Molecular Weight:
238.30
UNSPSC Code:
12352201
NACRES:
NA.31
PubChem Substance ID:
EC Number:
206-703-0
Beilstein/REAXYS Number:
4631
MDL number:
제품 이름
IPTG, ≥99% (TLC), ≤0.1% Dioxane
InChI key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
SMILES string
CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
biological source
synthetic
description
non-ionic
assay
≥99% (TLC)
form
powder
technique(s)
thin layer chromatography (TLC): suitable
impurities
≤0.1% Dioxane
color
white to off-white
useful pH range
5-7 (50 g/L)
mp
105 °C ((221 °F))
solubility
water: 0.05 g/L, clear
application(s)
cell analysis
life science and biopharma
storage temp.
2-8°C
Quality Level
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Application
IPTG has been used:
- in the induction of protein expression in Lactobacillus reuteri GroEL and mutants
- in the induction of protein expression in Escherichia coli
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Biochem/physiol Actions
IPTG is a non-metabolizable analog of allolactose. It acts as an inducer that regulates the transcriptional activity of the lac promoter, a promoter that is used for the expression of the protein in E.coli.
General description
Isopropyl β-D-Thiogalactopyranoside (IPTG) is a molecular biology reagent used to induce gene expression in bacterial systems. It mimics lactose, binding to the lac repressor protein, leading to gene activation. IPTG is crucial for studying gene regulation, protein expression, and recombinant protein production, providing valuable insights into cellular and molecular biology research.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Non-metabolizable galactose analog.
To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Preparation Note
A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B
저장 등급
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Bin Jiang et al.
Molecular metabolism, 59, 101462-101462 (2022-03-06)
Chronic inflammatory response plays a prominent role in obesity-related nonalcoholic fatty liver disease (NAFLD). However, the intrahepatic triggering mechanism of inflammation remains obscure. This study aimed to elucidate the role of serum amyloid A1 (SAA1), an acute-phase response protein, in
Alexandre M M Dias et al.
Journal of gastroenterology, 56(5), 442-455 (2021-03-31)
We previously showed that supernatants of Lactobacillus biofilms induced an anti-inflammatory response by affecting the secretion of macrophage-derived cytokines, which was abrogated upon immunodepletion of the stress protein GroEL. We purified GroEL from L. reuteri and analysed its anti-inflammatory properties
A self-inducible heterologous protein expression system in Escherichia coli
Briand L, et al.
Scientific Reports, 6 , 33037 -33037 (2016)
Prajna Jena et al.
Nanoscale, 12(6), 3731-3749 (2020-01-30)
The actin cytoskeleton is required for the maintenance of the cell shape and viability of bacteria. It remains unknown to which extent nanoparticles (NPs) can orchestrate the mechanical instability by disrupting the cytoskeletal network in bacterial cells. Our work demonstrates
João Paulo Fabi et al.
PloS one, 9(8), e105685-e105685 (2014-08-28)
Papaya (Carica papaya L.) is a climacteric fleshy fruit that undergoes dramatic changes during ripening, most noticeably a severe pulp softening. However, little is known regarding the genetics of the cell wall metabolism in papayas. The present work describes the
문서
General protocols for growth of competent cells and their transformation (uptake of DNA).
프로토콜
General protocols for growth of competent cells in microbial medium.
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