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Merck

N6639

Nitrotetrazolium Blue chloride

powder, electrophoresis grade

동의어(들):

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
C40H30N10O6 · 2Cl
CAS 번호:
298-83-9
Molecular Weight:
817.64
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
24897786
EC Number:
206-067-4
Beilstein/REAXYS Number:
4115923
MDL number:
MFCD00012159

주요 문서

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InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

grade

electrophoresis grade

assay

>90%

form

powder

technique(s)

electrophoresis: suitable

solubility

H2O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.), 2-methoxyethanol: 20 mg/mL, ethanol: 5 mg/mL

storage temp.

2-8°C

Quality Level

100

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General description

Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).

Application

Nitrotetrazolium Blue chloride has been used:
  • to determine the superoxide anion in skeletal muscle cells
  • as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sections
  • as a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O2- ) anion in Brassica juncea seedlings
  • as a component in Triton X-100 solution to perform staining of coronal brain sections

Biochem/physiol Actions

Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O2.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SHBT1736
SHBR8150
SHBQ4267
SHBR1013
SHBN5587

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Low Intensity Exercise Training Improves Skeletal Muscle Regeneration Potential
Pietrangelo T, et al.
Frontiers in Physiology, 6 (2015)
Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex
Liu C, et al.
Frontiers in Neuroanatomy, 9 (2015)
Histochemical detection of superoxide and H2O2 accumulation in Brassica juncea seedlings
Kumar D, et al.
Bio-protocol, 4(8), e1108-e1108 (2014)
The systemDrive: a Multisite, Multiregion Microdrive with Independent Drive Axis Angling for Chronic Multimodal Systems Neuroscience Recordings in Freely Behaving Animals
Billard MW, et al.
eNeuro, 5(6) (2018)
Chihiro Ebihara et al.
Human molecular genetics, 24(15), 4238-4249 (2015-05-03)
Seipin, encoded by BSCL2 gene, is a protein whose physiological functions remain unclear. Mutations of BSCL2 cause the most-severe form of congenital generalized lipodystrophy (CGL). BSCL2 mRNA is highly expressed in the brain and testis in addition to the adipose
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