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Merck

P7170

Ponceau S solution

BioReagent, 0.1 % (w/v) in 5% acetic acid

동의어(들):

Ponceau S

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C22H12N4Na4O13S4
CAS 번호:
Molecular Weight:
760.57
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
MDL number:
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제품 이름

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

description

for protein analysis

product line

BioReagent

form

liquid

concentration

0.1 % (w/v) in 5% acetic acid

technique(s)

western blot: suitable

density

1.01 g/cm3 at 20 °C

suitability

suitable (for use in cellulose acetate electrophoresis)
suitable for electrophoresis

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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Application

Ponceau S solution has been used for membrane staining (Western blotting).

General description

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

저장 등급

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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문서

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

프로토콜

Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.

다양한 블롯 절차를 위한 워크플로 단계를 가진 웨스턴 블롯 프로토콜은 단백질이 SDS 폴리아크릴아미드 겔에서 니트로셀룰로오스 시트로 전기영동을 통해 전달되는 과정을 설명합니다. 이는 또한 면역 블로팅 프로토콜로도 알려져 있습니다.

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