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Merck

27815

Brilliant Blue G

250, suitable for microscopy

동의어(들):

Acid blue 90, Coomassie Brilliant Blue G

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C47H48N3NaO7S2
CAS 번호:
Molecular Weight:
854.02
EC Number:
228-058-4
UNSPSC Code:
12171500
NACRES:
NA.26
PubChem Substance ID:
MDL number:
Colour Index Number:
42655
Beilstein/REAXYS Number:
5230822
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InChI key

RWVGQQGBQSJDQV-UHFFFAOYSA-M

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

form

solid

technique(s)

microbe id | staining: suitable

suitability

suitable for microscopy

Quality Level

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관련 카테고리

General description

Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.

Application

Brilliant Blue G was used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery.

Biochem/physiol Actions

P2X7 purinergic receptor antagonist.

Analysis Note

λmax. ∼610 nm; E(1%,1 cm) ∼500-700 (H2O)

Other Notes

Protein stain for gel electrophoresis. Does not penetrate the gel and is three times more sensitive than Naphthol Blue Black B; Simple and sensitive protein assay; Rapid, sensitive and versatile assay for proteins; free amino acids and several chemicals that interfere with the Lowry procedure cause no interference.

Legal Information

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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시험 성적서(COA)

Lot/Batch Number

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Isolation and activation of collagenase from fish processing waste.
Daboor SM, et al.
Advances in Bioscience and Biotechnology, 3(3) (2012)
M Remy et al.
The British journal of ophthalmology, 92(8), 1142-1147 (2008-07-26)
To evaluate the retinal toxicity of Brilliant Blue G (BBG) following intravitreal injection in rat eyes and examine the biocompatibility and the staining properties in humans. BBG was injected into the 11 rat eyes to evaluate toxic effects with balanced
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.
J J Sedmak et al.
Analytical biochemistry, 79(1-2), 544-552 (1977-05-01)
W.J. Shaphonov
Methods in Enzymology, 11, 184-184 (1967)
Hiroshi Enaida et al.
Retina (Philadelphia, Pa.), 26(6), 631-636 (2006-07-11)
To report the use of the dye brilliant blue G (BBG) for staining of the internal limiting membrane (ILM) during macular hole (MH) and epiretinal membrane (ERM) surgery. This study was designed as an interventional, noncomparative, prospective, clinical case series.

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