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32670

4′,6-Diamidino-2-phenylindole dihydrochloride

Name: 4′,6-Diamidino-2-phenylindole dihydrochloride
Brand: SIGMA

suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

동의어(들):

2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

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크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):

C₁₆H₁₅N₅ · 2HCl

CAS 번호:

28718-90-3

Molecular Weight:

350.25

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

57648699

EC Number:

249-186-7

Beilstein/REAXYS Number:

4894417

MDL number:

MFCD00012681

기술 서비스

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도움 문의

속성

product line

BioReagent

Quality Level

100

assay

≥95.0% (HPLC)

form

powder

solubility

DMF: soluble, H₂O: soluble

fluorescence

λ_ex 340 nm; λ_em 488 nm (nur DAPI), λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex), λ_ex 374 nm; λ_em 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA

suitability

suitable for fluorescence

storage temp.

room temp

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

설명

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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pictograms

GHS07

signalword

Warning

hcodes

H315,H317,H335

pcodes

P261 - P264 - P271 - P272 - P280 - P302 + P352

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

target_organs

Respiratory system

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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