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Merck

36798

7-(Diethylamino)coumarin-3-carbohydrazide

BioReagent, suitable for fluorescence, ≥95% (HPCE)

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C14H17N3O3
CAS 번호:
Molecular Weight:
275.30
UNSPSC Code:
12352125
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5574454
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product line

BioReagent

assay

≥95% (HPCE)

solubility

DMF: soluble, acetonitrile: soluble, chloroform: soluble, methanol: soluble

fluorescence

λex 450 nm; λem 468 nm in methanol

suitability

suitable for fluorescence

SMILES string

CCN(CC)c1ccc2C=C(C(=O)NN)C(=O)Oc2c1

InChI

1S/C14H17N3O3/c1-3-17(4-2)10-6-5-9-7-11(13(18)16-15)14(19)20-12(9)8-10/h5-8H,3-4,15H2,1-2H3,(H,16,18)

InChI key

LYBMHAINSFEHRL-UHFFFAOYSA-N

General description

7-(Diethylamino)coumarin-3-carbohydrazide is a coumarin-hydrazide reagent typically used as a fluorescent chemical probe for labeling of cellular carbonyls.

Application

7-(Diethylamino)coumarin-3-carbohydrazide is used for derivatization and detection of carboxylic acid and carbonylated molecules such as lipid peroxidation products (LPP) by electrospray ionization-mass spectrometry (ESI-MS) in positive ion mode. It may be used as a fluorescent label for the determination of lipid aldehydes in food products by normal phase liquid chromatography (NPLC) coupled to ESI-MS and fluorescent detector (FLD) and for the derivatization of monoterpene alcohols in urine prior to analysis by high performance liquid chromatography(HPLC) with FLD coupled to ESI-MS.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivatizing agent for carboxylic acid detection

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Roland J W Meesters et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 875(2), 444-450 (2008-10-11)
A method was developed for the determination of the monoterpene alcohols verbenol, myrtenol, perillyl alcohol, alpha-terpineol, Delta(3)-carene-10-ol, thymol and p-alpha,alpha-trimethylbenzylalcohol in urine samples. After an enzymatic cleavage of their glucuronide- and sulfate conjugates the monoterpene alcohols were converted in the
Ivana Milic et al.
Analytical chemistry, 85(1), 156-162 (2012-11-29)
Reactive oxygen species (ROS) and other oxidative agents such as free radicals can oxidize polyunsaturated fatty acids (PUFA) as well as PUFA in lipids. The oxidation products can undergo consecutive reactions including oxidative cleavages to yield a chemically diverse group
M.L. Grayeski et al.
Analytical Chemistry, 59, 1203-1203 (1987)
Simultaneous detection of low and high molecular weight carbonylated compounds derived from lipid peroxidation by electrospray ionization-tandem mass spectrometry
Milic I, et al.
Analytical Chemistry, 85(1), 156-162 (2012)
Thomas J Nelson
Analytical biochemistry, 419(1), 40-45 (2011-08-30)
A new ultrasensitive fluorescent derivatization procedure for chromatographic analysis of primary, secondary, and nonpolar tertiary alcohols is described. The procedure uses Bodipy FL in basic dichloromethane solution with Mukaiyama's reagent (2-chloro-1-methylpyridinium iodide) to form highly fluorescent ester derivatives that can

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