83264
HEPES solution
1 M in H₂O, liquid
동의어(들):
HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12161700
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25
MDL number:
제품 이름
HEPES buffer solution, 1 M in H2O
SMILES string
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
form
liquid
concentration
1 M in H2O
238 g/L HEPES in H2O
impurities
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
pH
5.5±0.5
useful pH range
6.8-8.2
density
1.07 g/mL at 20 °C
application(s)
diagnostic assay manufacturing
Quality Level
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Application
HEPES has been used:
- As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
- To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
- As a component of dissection buffer for culturing rat cortical neurons
- Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
- As a component of Danieu′s aquarium water used in agarose gel preparation
General description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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