A2172
Anti-α-Actin-1 (ACTA1) antibody
mouse monoclonal, 5C5
동의어(들):
Mouse Anti-Actin
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
5C5, monoclonal
Application:
ELISA (i), IHC (f), IHC (p), WB
Citations:
215
제품 이름
Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse, clone 5C5, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
5C5, monoclonal
contains
15 mM sodium azide
species reactivity
rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle, immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle, indirect ELISA: suitable, western blot: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTA1(58), ACTC1(70)
mouse ... Rwdd2a(69519)
rat ... Acta1(29437), Actc1(29275)
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General description
Monoclonal Anti-α-Sarcomeric Actin (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins and the isoforms show >90% overall sequence homology. It is expressed in mammals and birds as six isoforms. Four of the six represent differentiation markers of muscle tissues. The other two are found in practically all cells.
Immunogen
rabbit striated muscle actin.
Application
Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has also been used
- immunofluorescence
- double immunostaining
- double labelling terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunofluorescence staining.
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications.
Biochem/physiol Actions
Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.
Physical form
The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
PEDF and PEDF-derived peptide 44mer stimulate cardiac triglyceride degradation via ATGL
Zhang H, et al.
Journal of Translational Medicine, 13(1), 68-68 (2015)
PEDF improves cardiac function in rats subjected to myocardial ischemia/reperfusion injury by inhibiting ROS generation via PEDF-R
Zhao Q, et al.
International Journal of Molecular Medicine, 41(6), 3243-3252 (2018)
Forced myocardin expression enhances the therapeutic effect of human mesenchymal stem cells after transplantation in ischemic mouse hearts
Grauss RW, et al.
Stem Cells, 26(4), 1083-1093 (2008)
Xiao Gao et al.
Scientific reports, 4, 5637-5637 (2014-07-12)
Pigment epithelium-derived factor (PEDF) has many biological activities. But it's not known whether PEDF and its functional peptides could protect against hypoxia-induced cell death and the mechanisms are still unclear. We used cultured H9c2 cells and primary cardiomyocytes to show
Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle
Chaponnier C and Gabbiani G
F1000Research, 5 (2016)
관련 콘텐츠
Datasheet
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