제품 이름
Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1
sterility
0.2 μm filtered
Quality Level
product line
BioReagent
form
liquid
feed ratio
29:1
technique(s)
electrophoresis: suitable
suitability
suitable for electrophoresis
foreign activity
Protease, none detected
storage temp.
2-8°C
SMILES string
NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
InChI
1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
InChI key
OCQZXMCGTAWGEQ-UHFFFAOYSA-N
General description
Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.
Application
These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 4% stacking gel and 10% resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis. It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods.
Preparation Note
Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
target_organs
Peripheral nervous system
저장 등급
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
관련 콘텐츠
Instructions
Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Chen Q, et al.
Bio-protocol, 3(22) (2013)
Subcellular western blotting of single cells
Yamauchi KA and Herr AE
Microsystems & Nanoengineering, 3, 16079-16079 (2017)
Ana Techniqs in Biotechnology (2011)

