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Merck

A5228

Anti-α-Smooth Muscle Actin (ACTA2) Antibody,

mouse monoclonal, 1A4

동의어(들):

Monoclonal Anti-Actin, α-Smooth Muscle, Acta2 Antibody, Alpha Smooth Muscle Actin Antibody Sigma, SMA Antibody - Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal, Sma Antibody, alpha SMA, SMA

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41

주요 문서

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제품 이름

Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal, clone 1A4, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A4, monoclonal

form

PBS solution

mol wt

antigen ~42 kDa

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable using smooth muscle cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
indirect ELISA: suitable
indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections
microarray: suitable
western blot: suitable using smooth muscle cells

isotype

IgG2a

UniProt accession no.

P62736

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

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Application

Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal has been used in:
  • immunofluorescence staining
  • immunohistochemistry
  • immunoblotting
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)
  • western blotting
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Biochem/physiol Actions

Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, cytokinesis, secretion, cytoplasmic streaming and phagocytosis. It has been shown that the relative proportion of actin isoforms are different in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions. The actin in cells of various species and tissue origin are very similar in their immunological and physical properties.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle).
Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the NH2 terminal synthetic decapeptide of a smooth muscle actin, coupled to keyhole limpet hemocyanin (KLH).
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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저장 등급

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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시험 성적서(COA)

Lot/Batch Number
0000504551
0000504550
0000504551
0000504550
0000519672

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문서 라이브러리 방문

Danna M Breen et al.
Arteriosclerosis, thrombosis, and vascular biology, 29(7), 1060-1066 (2009-04-11)
Insulin has both growth-promoting and protective vascular effects in vitro, however the predominant effect in vivo is unclear. We investigated the effects of insulin in vivo on neointimal growth after arterial injury. Rats were given subcutaneous control (C) or insulin
Jung-Yeon Kim et al.
International journal of molecular medicine, 39(5), 1188-1194 (2017-04-14)
Cholestatic liver disease is characterized by the progressive destruction of biliary epithelial cells (BECs) followed by fibrosis, cirrhosis and liver failure. Activated hepatic stellate cells (HSCs) and portal fibroblasts are the major cellular effectors of enhanced collagen deposition in biliary fibrosis.
Induction of renal fibrotic genes by TGF-beta1 requires EGFR activation, p53 and reactive oxygen species
Samarakoon R, et al.
Cellular Signalling, 25(11), 2198-2209 (2013)
Neuron-like differentiation of adipose tissue-derived stromal cells and vascular smooth muscle cells
Ning H, et al.
Differentiation, 74(9-10), 510-518 (2006)
Risk of dissection in thoracic aneurysms associated with mutations of smooth muscle alpha-actin 2 (ACTA2)
Disabella E, et al.
Heart, 97(4), 321-326 (2011)
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Data Sheet - A5228

Instructions

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