A5691
Anti-Actin, α-Smooth Muscle - Alkaline Phosphatase antibody, Mouse monoclonal
clone 1A4, purified from hybridoma cell culture
동의어(들):
Monoclonal Anti-Actin, α-Smooth Muscle, Monoclonal Anti-Actin, α-Smooth Muscle - Alkaline Phosphatase antibody produced in mouse, SMA
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
1A4, monoclonal
Application:
ELISA
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot
Technique(s):
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
Citations:
77
Uniprot accession no.:
Species reactivity:
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
제품 이름
Anti-Actin, α-Smooth Muscle - Alkaline Phosphatase antibody, Mouse monoclonal, clone 1A4, purified from hybridoma cell culture
biological source
mouse
conjugate
alkaline phosphatase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
1A4, monoclonal
form
buffered aqueous glycerol solution
mol wt
antigen ~42 kDa
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
isotype
IgG2a
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Acta2(11475)
rat ... Acta2(81633)
species reactivity
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
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Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Immunocytochemistry was performed on kertocytes fixed in 1% PFA and incubated with mouse monoclonal anti-smooth muscle actin (14A) at a 1:100 dilution.
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Mouse monoclonal anti-actin, α−smooth muscle-alkaline phosphatase antibody has been used for immunohistochemical application in mouse and human tissues.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
Immunogen
N-terminal synthetic decapeptide of α-smooth muscle actin.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide as a preservative.
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저장 등급
10 - Combustible liquids
wgk
WGK 2
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Florence Morin et al.
Journal of immunology (Baltimore, Md. : 1950), 197(8), 3018-3028 (2016-09-11)
Systemic sclerosis (SSc) is a connective tissue disorder characterized by fibrosis of the skin and inner organs, vasculopathy, and immunological abnormalities. Recent insights on the implication of STAT3, AKT, and Wnt/β-catenin in fibrosis have prompted us to investigate, in a
Immunohistochemical analysis of Sonic hedgehog signalling in normal human urinary tract development.
Dagan Jenkins et al.
Journal of anatomy, 211(5), 620-629 (2007-09-14)
Studies of mouse mutants have demonstrated that Sonic hedgehog (SHH) signalling has a functional role in morphogenesis and differentiation at multiple sites within the forming urinary tract, and urinary tract malformations have been reported in humans with mutations that disrupt
Alex H P Chan et al.
PloS one, 12(3), e0174773-e0174773 (2017-03-30)
Current animal models for the evaluation of synthetic grafts are lacking many of the molecular tools and transgenic studies available to other branches of biology. A mouse model of vascular grafting would allow for the study of molecular mechanisms of
Maryellen Sandor et al.
Eplasty, 17, e1-e1 (2017-01-26)
Objective: Benchtop methods were evaluated for preclinical inflammation/capsule formation correlation following implantation of human acellular dermal matrices. Methods: Dermal matrices were compared with native dermis for structure (histology, scanning electron microscopy), collagen solubility (hydroxyproline), enzymatic susceptibility (collagenase), and thermal stability
Kenneth Chan et al.
Journal of the American Heart Association, 6(11) (2017-11-02)
Genome-wide association studies identified ADAMTS7 as a risk locus for coronary artery disease (CAD). Functional studies suggest that ADAMTS7 may promote cellular processes in atherosclerosis. We sought to examine the association between genetic variation at ADAMTS7 and measures of atherosclerosis
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