B1522
Basal Medium Eagle
With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
동의어(들):
BME
제품 이름
Basal Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
Eagle′s Basal Medium (BME) is one of the most used synthetic cell culture media. The tissue culture association recommends that the term "Eagle′s Basal Medium" be used only to refer to the formula developed to support HeLa cells. BME has previously been used in monolayer culture studies to assess the growth response of normal (WI-38) and transformed (mouse and HeLa) cells. BME is the precursor of Dulbecco′s Modified Eagle′s medium (DME) and Eagle′s Minimum Essential Medium (MEM).
Application
Basal Medium Eagle has been used:
- as a component in the culture medium for cochlear organotypic cultures and salicylate treatment
- to maintain cerebellar granule neurons (CGNs) to determine if there is a system that regulates the distribution and/or group dynamics of axonal mitochondria
- in the preparation of cortical slices, serum-free medium, and slice culture medium for slice overlay assay to study the influence of extracellular signals on neuronal development
Preparation Note
Supplement with 0.292 g/L L-glutamine.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Franck Polleux et al.
Science's STKE : signal transduction knowledge environment, 2002(136), pl9-pl9 (2002-06-13)
We have developed a technique that allows characterization and identification of extracellular signals that regulate various aspects of neuronal differentiation. In this in vitro assay, dissociated cells isolated from the developing cerebral wall are labeled and cultured over organotypic cortical
Intermitochondrial signaling regulates the uniform distribution of stationary mitochondria in axons.
Nozomu Matsumoto et al.
Molecular and cellular neurosciences, 119, 103704-103704 (2022-02-09)
In the central nervous system (CNS), many neurons develop axonal arbors that are crucial for information processing. Previous studies have demonstrated that premature axons contain motile and stationary mitochondria, and their balance is important for axonal arborization. However, the mechanisms
L Wei et al.
Neuroscience, 168(1), 288-299 (2010-03-20)
Aspirin, whose active ingredient is sodium salicylate, is the most widely used drug worldwide, but it is not recommended for children because it may cause Reye's syndrome. High doses of salicylate also induce temporary hearing loss and tinnitus; while these
Bethtrice Thompson et al.
PloS one, 11(2), e0148619-e0148619 (2016-02-20)
Bone continuously undergoes remodeling by a tightly regulated process that involves osteoblast differentiation from Mesenchymal Stem Cells (MSC). However, commitment of MSC to osteoblastic lineage is a poorly understood process. Chromatin organization functions as a molecular gatekeeper of DNA functions.
G M Seigel et al.
Molecular and cellular neurosciences, 85, 202-210 (2017-11-08)
Microglia are glial-immune cells that are essential for the function and survival of the central nervous system. Microglia not only protect neural tissues from immunological insults, but also play a critical role in neural development and repair. However, little is
문서
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Cell culture protocol for passaging and splitting suspension cell lines
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