B4287
Bovine Serum Albumin
heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%
동의어(들):
Albumin bovine serum, BSA, Bovine albumin
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, electrophoresis: suitable, western blot: suitable
Impurities:
protease, free
biological source
bovine
grade
Molecular Biology
product line
BioReagent
assay
≥98%
form
lyophilized powder
Quality Level
Gene Information
bovine ... ALB(280717)
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable, electrophoresis: suitable, western blot: suitable
impurities
protease, free
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
Protease free
storage temp.
2-8°C
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Application
A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Protease free
- Suitable for blocking non-specific protein binding sites in hybridizations
- Heat shock fractionated
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Salmonella enterica serovar Typhi can establish a chronic, asymptomatic infection of the human gallbladder, suggesting that this bacterium utilizes novel mechanisms to mediate enhanced colonization and persistence in a bile-rich environment. Gallstones are one of the most important risk factors
프로토콜
The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.
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