General description
Protein determination is one of the most common operations performed in biochemical research. Bicinchoninic acid solution is used in the bicinchoninic acid (BCA) assay, which is equivalent to the Lowry procedure. Both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. The BCA assay is more sensitive and applicable than either biuret or Lowry procedures.
Application
This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.
Bicinchoninic Acid solution (Bicinchoninic acid protein assay kit) is used :
- For the determination of total protein content in the process of quantifying bromelain, extracted from the pineapple stem residues using aqueous micellar two-phase systems composed of ionic liquids as co-surfactants.
- For the determination of bovine serum albumin (BSA) in biomimetic calcium phosphate coating co-precipitated on titanium alloy plates.
Features and Benefits
- It is easy to use.
- The color complex is stable.
- There is less susceptibility to detergents.
- It is applicable over a broad range of protein concentrations.
Packaging
Also available as part of the Bicinchoninic Acid Kit for Protein Determination (BCA-1).
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Warning
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pcodes
Hazard Classifications
Met. Corr. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
저장 등급
12 - Non Combustible Liquids
프로토콜
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
관련 콘텐츠
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
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