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Merck

BCA1

Bicinchoninic Acid Kit for Protein Determination

for 200-1000 μg/ml protein

동의어(들):

BCA protein assay

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.32
UNSPSC Code:
12352106

주요 문서

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제품 이름

Bicinchoninic Acid Kit for Protein Determination, for 200-1000 μg/ml protein

usage

sufficient for ~500 assays

storage temp.

2-8°C

Quality Level

200

Application

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.

Biochem/physiol Actions

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Features and Benefits

  • Simple, sensitive colorimetric assay for proteins
  • Two stable reagents are used for the working solution
  • Faster and easier than the Lowry protein assay
  • Compatible with many detergents both ionic and nonionic
  • Less variation between different proteins than the Bradford dye-binding assay
  • Adaptable for use in microwell plates

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

키트 구성품 전용

제품 번호
설명
  • Bicinchoninic Acid Solution 1 L
  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL
  • Protein Standard Solution 5 x 1

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

저장 등급

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000502790
0000502807
0000502807
0000502790
0000484749

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문서 라이브러리 방문

D Jantas et al.
Neurotoxicity research, 25(2), 208-225 (2013-10-10)
Tianeptine (Tian) possesses neuroprotective potential, however, little is known about the effect of this drug in models of neuronal apoptosis. In the present study, we aimed (1) to compare the neuroprotective capacities of some antidepressants (ADs) in the models of
TGFB1 modulates in vitro secretory activity and viability of equine luteal cells
Galvao A, et al.
Cytokine, 110, 316-327 (2018)
Human OATP1B1 (SLCO1B1) transports sulfated bile acids and bile salts with particular efficiency
Toth B, et al.
Toxicology in vitro, 52, 189-194 (2018)
Salivary proteome patterns of individuals exposed to High Altitude
Jain S, et al.
Archives of Oral Biology, 96, 104-112 (2018)
R E Brown et al.
Analytical biochemistry, 180(1), 136-139 (1989-07-01)
Protein quantitation based on bicinchoninic acid (BCA) is simple, sensitive, and tolerant to many detergents and substances known to interfere with the Lowry method. However, certain compounds often used during protein purification do interfere with the BCA protein assay. The
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문서

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

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